DNA in situ hybridization as a diagnostic tool in the discrimination of melanoma and spitz naevus
Article first published online: 15 JUN 2005
Copyright © 1994 John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 173, Issue 3, pages 227–233, July 1994
How to Cite
de wit, P. E. J., Kerstens, H. M. J., Poddighe, P. J., Van Muijen, G. N. P. and Ruiter, D. J. (1994), DNA in situ hybridization as a diagnostic tool in the discrimination of melanoma and spitz naevus. J. Pathol., 173: 227–233. doi: 10.1002/path.1711730305
- Issue published online: 15 JUN 2005
- Article first published online: 15 JUN 2005
- Manuscript Accepted: 24 APR 1994
- Manuscript Received: 14 FEB 1994
- EORTC Melanoma Cooperative Group
- Spitz naevus;
- in situ hybridization;
- chromosome aberrations;
As the clinical and histological differential diagnosis between Spitz naevus and cutaneous melanoma may be very difficult, we have investigated whether DNA in situ hybridization may be helpful in resolving this problem. To this end, routinely-processed paraffin sections of 15 typical Spitz naevi, 15 typical nodular melanomas, and five cases originally misdiagnosed as Spitz naevi but which later metastasized and were reclassified as melanoma were analysed using a method previously described (De Wit et al., J Invest Dermatol 1992; 98: 450–458). Microscopical semi-quantitative evaluation revealed that the number of nuclei with supernumerary aberrations of the centromere region of chromosome 1, suggestive of aneuploidy, was significantly different in Spitz naevi and nodular melanoma. The mean number of aberrant nuclei per high power field was 0.41 and 4.01, respectively (P=0.0001). On applying the results of the typical lesions to the equivocal, originally misdiagnosed lesions, three out of five could be identified as melanoma. These results suggest that the application of DNA in situ hybridization may contribute to the positive identification of histologically equivocal pigmented lesions. The advantages of this technique are that it is cheap, requires little tissue, and can be applied on routinely-processed paraffin sections.