Recombination pattern of the TCR γ locus in human peripheral T-cell lymphomas
Article first published online: 15 JUN 2005
Copyright © 1994 John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 174, Issue 4, pages 233–242, December 1994
How to Cite
Theodorou, J., Raphaël, M., Bigorgne, C., Fourcade, C., Lahet, C., Cochet, G., Lefranc, M.-P., Gaulard, P. and Farcet, J.-P. (1994), Recombination pattern of the TCR γ locus in human peripheral T-cell lymphomas. J. Pathol., 174: 233–242. doi: 10.1002/path.1711740402
- Issue published online: 15 JUN 2005
- Article first published online: 15 JUN 2005
- Manuscript Accepted: 14 JUL 1994
- Manuscript Received: 6 MAY 1994
- Institut National de la Santé et de la Recherche Médicale
- Association de la Recherche contre le Cancer
- Université Paris-val-Marne. I.T.
- T-cell receptor;
- peripheral T-cell lymphoma;
- γ gene;
- β gene;
- δ gene;
- Southern blot;
- PCR gene rearrangement
The recombination events of the γ and β T-cell receptor (TCR) loci were analysed in a series of 39 peripheral T-cell lymphomas (PTCLs) in association with the expression of TCR chains. In TCR αβ PTCLs, 22/23 cases showed a γ-gene rearrangement while only 18/23 showed a concomitant β-gene rearrangement. The germline configuration of the β locus was found in angioimmunoblastic lymphadenopathy and lymphoepithelioid lymphomas. Three γδ PTCLs rearranged both γ and β genes. TCR silent PTCLs showed three different patterns of γ- and β-gene rearrangements. Three cases were in germline configuration for both loci; five cases had a rearranged γ and a germline β locus; and five cases had the two loci rearranged. Regarding the variable genes in the γ-rearranged alleles, members of the VγI subgroup were the most frequently presented (39/50), followed by VγII, VγIII, and VγIV (9/50, 1/50, and 1/50, respectively). Joining segment usage was as follows: J1 or J2 (32/50), JP1 or JP2 (17/50), and JP (1/50). Taken together, these data demonstrate that the γ locus is more frequently rearranged whatever the TCR expression. The γ-locus analysis provides a better diagnostic yield than the β locus in the study of PTCL clonality.