Differentiation of a human rhabdomyosarcoma cell line after antineoplastic drug treatment

Authors

  • C. Melguizo,

    1. Basic Cardiovascular Research Section, Department of Morphological Sciences, School of Medicine, University of Granada, E-18071 Granada, Spain
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  • J. Prados,

    1. Basic Cardiovascular Research Section, Department of Morphological Sciences, School of Medicine, University of Granada, E-18071 Granada, Spain
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  • J. Aneiros,

    1. Department of Pathology, School of Medicine, University of Granada, E-18071 Granada, Spain
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  • J. E. Fernandez,

    1. Basic Cardiovascular Research Section, Department of Morphological Sciences, School of Medicine, University of Granada, E-18071 Granada, Spain
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  • C. Velez,

    1. Basic Cardiovascular Research Section, Department of Morphological Sciences, School of Medicine, University of Granada, E-18071 Granada, Spain
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  • A. Aranega

    Corresponding author
    1. Basic Cardiovascular Research Section, Department of Morphological Sciences, School of Medicine, University of Granada, E-18071 Granada, Spain
    • Sección de Investigación Básica Cardiovascular, Dept. de Ciencias Morfológicas, Facultad de Medicina, Universidad de Granada, E-18071 Granada, Spain
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Abstract

The feasibility of treating solid tumours with differentiation therapy using antineoplastic drugs is currently being investigated, but the emergence of multidrug resistance remains the major limitation to this therapeutic approach. A rhabdomyosarcoma cell line resistant to actinomycin D (RD-DAC) has been used as an in vitro model to investigate, with light and electron microscopy, the degree of differentiation in multidrug-resistant cells. The parental cell line (RD), derived from a human embryonic-type rhabdomyosarcoma, is undifferentiated, with no evidence of specific ultrastructural markers. Examination of resistant cells by transmission electron microscopy revealed myofilaments arranged parallel to the long axis of the cell, which was considered clear evidence of myogenic differentiation. These observations suggest that actinomycin D, the drug of choice in the treatment of rhabdomyosarcoma, induces differentiation in the cell line RD. It is postulated that multidrug resistance can interfere with cellular differentiation.

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