No conflicts of interest were declared.
The contributions of oestrogen receptor isoforms to the development of papillary and anaplastic thyroid carcinomas†
Article first published online: 11 DEC 2007
Copyright © 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 214, Issue 4, pages 425–433, March 2008
How to Cite
Zeng, Q., Chen, G., Vlantis, A., Tse, G. and van Hasselt, C. (2008), The contributions of oestrogen receptor isoforms to the development of papillary and anaplastic thyroid carcinomas. J. Pathol., 214: 425–433. doi: 10.1002/path.2297
- Issue published online: 8 FEB 2008
- Article first published online: 11 DEC 2007
- Accepted manuscript online: 11 DEC 2007 12:00AM EST
- Manuscript Accepted: 20 OCT 2007
- Manuscript Revised: 11 OCT 2007
- Manuscript Received: 3 JUL 2007
- Research Grants Council of the Hong Kong Special Administrative Region. Grant Number: CUHK4556/05M
- The Chinese University of Hong Kong. Grant Number: 2006.1.095
This article contains supplementary material available via the Internet from the Journal http://www.interscience.wiley.com/jpages/0022-3417/suppmat/path.2297.html
|path2297-PATH2297SuppFigs.docx||257K||Figure 1.Verification of subcellular fractions by anti-HSP90, a cytoplasmic marker; anti-prohibitin, a mitochondrial marker. Proteins isolated from the cytoplasm, the mitochondria and the nucleus of KAT5 and FRO cells reacted with the corresponding antibody for Western blot. The experiments were repeated three times on the three batch samples of each fraction.|
|path2297-PATH2297SuppFigs.docx||257K||Figure 2.Confirmation of the subcellular distribution of ER isoforms in KAT5 and FRO cells using DAPI. Cells were incubated with DAPI, fixed in 2% paraformaldehyde and treated with antibody against ERa, followed by incubation with rhodamine-conjugated secondary antibody and ERa was located in the nucleus. The experiment was repeated three times, using cells from independent cell cultures. DAPI staining: 1,4; ERa staining: 2,5; merged images:|
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