These authors contributed equally to the study.
Glioblastoma stem cells produce vascular endothelial growth factor by activation of a G-protein coupled formylpeptide receptor FPR†
Article first published online: 26 MAR 2008
Copyright © 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 215, Issue 4, pages 369–376, August 2008
How to Cite
Yao, X.-H., Ping, Y.-F., Chen, J.-H., Xu, C.-P., Chen, D.-L., Zhang, R., Wang, J. and Bian, X.-W. (2008), Glioblastoma stem cells produce vascular endothelial growth factor by activation of a G-protein coupled formylpeptide receptor FPR. J. Pathol., 215: 369–376. doi: 10.1002/path.2356
No conflicts of interest were declared.
- Issue published online: 4 JUL 2008
- Article first published online: 26 MAR 2008
- Accepted manuscript online: 26 MAR 2008 12:00AM EST
- Manuscript Accepted: 16 MAR 2008
- Manuscript Revised: 8 MAR 2008
- Manuscript Received: 11 DEC 2007
- National Basic Research Programme of China. Grant Number: 973 Project, No. 2006CB708503
- National Natural Science Foundation of China. Grant Number: 30670804
- cancer stem cells;
- formylpeptide receptor;
Glioma stem cells (GSCs), or stem cell-like glioma cells, isolated from malignant glioma cell lines, were capable of producing vascular endothelial growth factor (VEGF). However, the exact role of such tumour cells in angiogenesis remains unknown. In this study, we isolated a small proportion of CD133+ GSCs from the human glioblastoma cell line U87 and found that these GSCs possessed multipotent differentiation potential and released high levels of VEGF as compared with CD133− tumour cells. The CD133+ GSCs also formed larger xenograft tumours that contained higher VEGF immunoreactivity and denser microvessels. Moreover, GSCs expressed a functional G protein-coupled formylpeptide receptor FPR, which was activated by a chemotactic peptide ligand, N-formylmethionyl-leucyl-phenylalanine (fMLF), to mediate calcium flux and the production of VEGF by GSCs. Our results indicate that FPR expressed by human GSCs may play an important role in glioma angiogenesis. Copyright © 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.