No conflicts of interest were declared.
MicroRNA expression profiling of human metastatic cancers identifies cancer gene targets†
Version of Record online: 1 JUN 2009
Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 219, Issue 2, pages 214–221, October 2009
How to Cite
Baffa, R., Fassan, M., Volinia, S., O'Hara, B., Liu, C.-G., Palazzo, J. P., Gardiman, M., Rugge, M., Gomella, L. G., Croce, C. M. and Rosenberg, A. (2009), MicroRNA expression profiling of human metastatic cancers identifies cancer gene targets. J. Pathol., 219: 214–221. doi: 10.1002/path.2586
- Issue online: 10 SEP 2009
- Version of Record online: 1 JUN 2009
- Accepted manuscript online: 1 JUN 2009 12:00AM EST
- Manuscript Accepted: 25 MAY 2009
- Manuscript Revised: 20 MAY 2009
- Manuscript Received: 24 DEC 2008
- Benjamin Perkins Bladder Cancer Fund
- Martin Greitzer Fund
- metastatic cancers;
- gene target;
- expression signature
Small non-coding microRNAs (miRNAs) contribute to cancer development and progression, and are differentially expressed in normal tissues and cancers. However, the specific role of miRNAs in the metastatic process is still unknown. To seek a specific miRNA expression signature characterizing the metastatic phenotype of solid tumours, we performed a miRNA microarray analysis on 43 paired primary tumours (ten colon, ten bladder, 13 breast, and ten lung cancers) and one of their related metastatic lymph nodes. We identified a metastatic cancer miRNA signature comprising 15 overexpressed and 17 underexpressed miRNAs. Our results were confirmed by qRT-PCR analysis. Among the miRNAs identified, some have a well-characterized association with cancer progression, eg miR-10b, miR-21, miR-30a, miR-30e, miR-125b, miR-141, miR-200b, miR-200c, and miR-205. To further support our data, we performed an immunohistochemical analysis for three well-defined miRNA gene targets (PDCD4, DHFR, and HOXD10 genes) on a small series of paired colon, breast, and bladder cancers, and one of their metastatic lymph nodes. We found that the immunohistochemical expression of these targets significantly follows the corresponding miRNA deregulation. Our results suggest that specific miRNAs may be directly involved in cancer metastasis and that they may represent a novel diagnostic tool in the characterization of metastatic cancer gene targets. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.