These authors contributed equally to this work.
The cancer stem cell marker CD133 has high prognostic impact but unknown functional relevance for the metastasis of human colon cancer†
Article first published online: 25 JUN 2009
Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 219, Issue 4, pages 427–434, December 2009
How to Cite
Horst, D., Scheel, S. K., Liebmann, S., Neumann, J., Maatz, S., Kirchner, T. and Jung, A. (2009), The cancer stem cell marker CD133 has high prognostic impact but unknown functional relevance for the metastasis of human colon cancer. J. Pathol., 219: 427–434. doi: 10.1002/path.2597
No conflicts of interest were declared.
- Issue published online: 9 NOV 2009
- Article first published online: 25 JUN 2009
- Accepted manuscript online: 25 JUN 2009 12:00AM EST
- Manuscript Accepted: 18 JUN 2009
- Manuscript Revised: 29 MAY 2009
- Manuscript Received: 17 DEC 2008
- Friedrich-Baur-Stiftung, Germany. Grant Number: 0015/2007
- Wilhelm Sander-Stiftung, Germany. Grant Number: 2004-111.2
- knock down;
- colon cancer;
In colon cancer, CD133 has recently been used to enrich for a subset of tumour cells with tumour-initiating capabilities and was therefore suggested to mark colon cancer stem cells. However, this molecule has surprisingly been shown to lack functional importance for tumour initiation itself. Herein, we investigated whether CD133 may be relevant for colon cancer metastasis in patients, and as metastasis requires several additional biological characteristics besides tumour initiation, we examined the effects of knocking down CD133 expression in colon cancer cell lines on proliferation, migration, invasion, and colony formation. We demonstrate that high CD133 expression correlates strongly with synchronous liver metastasis in a matched case–control collection, while siRNA-mediated knock down of this factor has no significant effect on the mentioned biological characteristics. Thus, we conclude that CD133 expression is a marker with high prognostic impact for colon cancer, while it seems to have no obvious functional role as a driving force of this malignancy. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.