No conflicts of interest were declared.
Bioluminescent imaging: a critical tool in pre-clinical oncology research†
Article first published online: 27 OCT 2009
Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 220, Issue 3, pages 317–327, February 2010
How to Cite
O'Neill, K., Lyons, S. K., Gallagher, W. M., Curran, K. M. and Byrne, A. T. (2010), Bioluminescent imaging: a critical tool in pre-clinical oncology research. J. Pathol., 220: 317–327. doi: 10.1002/path.2656
- Issue published online: 26 JAN 2010
- Article first published online: 27 OCT 2009
- Accepted manuscript online: 27 OCT 2009 12:00AM EST
- Manuscript Accepted: 17 OCT 2009
- Manuscript Revised: 6 OCT 2009
- Manuscript Received: 8 JAN 2009
- bioluminescent imaging;
- animal models;
- non-invasive imaging
Bioluminescent imaging (BLI) is a non-invasive imaging modality widely used in the field of pre-clinical oncology research. Imaging of small animal tumour models using BLI involves the generation of light by luciferase-expressing cells in the animal following administration of substrate. This light may be imaged using an external detector. The technique allows a variety of tumour-associated properties to be visualized dynamically in living models. The increasing use of BLI as a small-animal imaging modality has led to advances in the development of xenogeneic, orthotopic, and genetically engineered animal models expressing luciferase genes. This review aims to provide insight into the principles of BLI and its applications in cancer research. Many studies to assess tumour growth and development, as well as efficacy of candidate therapeutics, have been performed using BLI. More recently, advances have also been made using bioluminescent imaging in studies of protein-protein interactions, genetic screening, cell-cycle regulators, and spontaneous cancer development. Such novel studies highlight the versatility and potential of bioluminescent imaging in future oncological research. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.