These authors contributed equally to the manuscript.
Article first published online: 14 SEP 2010
Copyright © 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 222, Issue 4, pages 400–409, December 2010
How to Cite
Willems, S. M., van Remoortere, A., van Zeijl, R., Deelder, A. M., McDonnell, L. A. and Hogendoorn, P. C. (2010), Imaging mass spectrometry of myxoid sarcomas identifies proteins and lipids specific to tumour type and grade, and reveals biochemical intratumour heterogeneity. J. Pathol., 222: 400–409. doi: 10.1002/path.2771
This article was published online on 14 September 2010. An error was subsequently identified. This notice is included in the online and print versions to indicate that both have been corrected [01 October 2010].
No conflicts of interest were declared.
- Issue published online: 25 OCT 2010
- Article first published online: 14 SEP 2010
- Accepted manuscript online: 20 AUG 2010 12:00AM EST
- Manuscript Accepted: 15 AUG 2010
- Manuscript Revised: 13 AUG 2010
- Manuscript Received: 29 JUL 2010
- Netherlands Organization for Scientific Research. Grant Number: 935-19-026
- ZonMW Agiko Stipendium. Grant Number: 920-03-403
- Re-use of this article is permitted in accordance with the Terms and Conditions set out at . [http://wileyonlinelibrary.com/onlineopen#OnlineOpen_Terms]
- myxoid liposarcoma;
- imaging mass spectrometry;
- clonal selection;
- tumour progression
Myxofibrosarcoma and myxoid liposarcomas are relatively common soft tissue tumours that are characterized by their so-called myxoid extracellular matrix and have to some extent overlap in histology. The exact composition and potential role of their myxoid extracellular matrix are insufficiently understood. To gain more insight into the biomolecular content of these tumours, we have studied 40 well-documented myxofibrosarcoma and myxoid liposarcoma cases using imaging mass spectrometry. This technique provides a multiplex biomolecular imaging analysis of the tissue, spanning multiple molecular domains and without a priori knowledge of the tissue's biomolecular content. We have developed experimental protocols for analysing the peptide, protein, and lipid content of myxofibrosarcoma and myxoid liposarcomas, and have detected proteins and lipids that are tumour-type and tumour-grade specific. In particular, lipid changes observed in myxoid liposarcomas could be related to pathways known to be affected during tumour progression. Unsupervised clustering of the biomolecular signatures was able to classify myxofibrosarcoma and myxoid liposarcomas according to tumour type and tumour grade. Closer examination of histologically similar regions in the tissues revealed intratumour heterogeneity, which was a consistent feature in each of the myxofibrosarcomas studied. In intermediate-grade myxofibrosarcoma, it was found that single tissue sections could contain regions with biomolecular profiles similar to high-grade and low-grade tumours, and that these regions were associated with the tumour's nodular structure, thus supporting a concept of tumour progression through clonal selection. Copyright © 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.