These authors contributed equally to this study.
Chronic chorioamnionitis displays distinct alterations of the amniotic fluid proteome†
Article first published online: 24 DEC 2010
Copyright © 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 223, Issue 4, pages 553–565, March 2011
How to Cite
Oggé, G., Romero, R., Lee, D.-C., Gotsch, F., Than, N. G., Lee, J., Chaiworapongsa, T., Dong, Z., Mittal, P., Hassan, S. S. and Kim, C. J. (2011), Chronic chorioamnionitis displays distinct alterations of the amniotic fluid proteome. J. Pathol., 223: 553–565. doi: 10.1002/path.2825
No conflicts of interest were declared.
- Issue published online: 3 FEB 2011
- Article first published online: 24 DEC 2010
- Accepted manuscript online: 23 NOV 2010 07:38AM EST
- Manuscript Accepted: 18 NOV 2010
- Manuscript Revised: 25 OCT 2010
- Manuscript Received: 4 AUG 2010
- Perinatology Research Branch, Division of Intramural Research
- Eunice Kennedy Shriver National Institute of Child Health and Human Development
- NIH, DHHS
- amniotic fluid;
- chronic chorioamnionitis;
- preterm birth
Acute chorioamnionitis of infectious origin and chronic chorioamnionitis of immunological origin are two major placental lesions of spontaneous preterm birth with elevated amniotic fluid interleukin-6 and CXCL10 concentrations, respectively. The changes in the amniotic fluid proteome associated with intra-amniotic infection and acute chorioamnionitis are well defined, yet alterations unique to chronic chorioamnionitis remain to be elucidated. This study was conducted to determine those amniotic fluid proteins changing specifically in the presence of chronic chorioamnionitis. Amniotic fluid obtained from acute chorioamnionitis, chronic chorioamnionitis and gestational age-matched controls were analysed by two-dimensional (2D) difference in gel electrophoresis and MALDI–TOF analyses. The type of histological inflammation was used to define each condition in preterm labour cases (n = 125) and term not in labour cases (n = 22), and the amniotic fluid concentrations of interleukin-6, CXCL8, CXCL10 and prostaglandin F2α were also measured by specific immunoassays. Among preterm labour cases, 31 differentially expressed proteins were identified in chronic chorioamnionitis cases as compared to both acute chorioamnionitis and control cases. Importantly, glycodelin-A, which maintains maternal tolerance against an allogeneic fetus, was decreased in chronic chorioamnionitis, while haptoglobin was increased. We report the amniotic fluid proteome of chronic chorioamnionitis for the first time, and the findings herein strongly suggest that there is a pathophysiological association between the changes of immunomodulatory proteins in the amniotic fluid and chronic chorioamnionitis, a histological manifestation of maternal anti-fetal allograft rejection. Copyright © 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.