These authors contributed equally to this study.
Article first published online: 9 DEC 2011
Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 226, Issue 3, pages 427–441, February 2012
How to Cite
Duprez, R., Wilkerson, P. M., Lacroix-Triki, M., Lambros, M. B., MacKay, A., Hern, R. A., Gauthier, A., Pawar, V., Colombo, P.-E., Daley, F., Natrajan, R., Ward, E., MacGrogan, G., Arbion, F., Michenet, P., Weigelt, B., Vincent-Salomon, A. and Reis-Filho, J. S. (2012), Immunophenotypic and genomic characterization of papillary carcinomas of the breast. J. Pathol., 226: 427–441. doi: 10.1002/path.3032
No conflicts of interest were declared.
Microarray comparative genomic hybridization data, the analysis history, script and code are available at: http://rock.icr.ac.uk/collaborations/ Mackay/Papillary.aCGH/
- Issue published online: 16 JAN 2012
- Article first published online: 9 DEC 2011
- Accepted manuscript online: 25 OCT 2011 04:52AM EST
- Manuscript Accepted: 12 OCT 2011
- Manuscript Revised: 21 SEP 2011
- Manuscript Received: 10 AUG 2011
- breast cancer;
- papillary carcinoma;
- comparative genomic hybridization;
- tissue microarrays;
- in situ hybridization
Papillary carcinomas are a special histological type of breast cancer and have a relatively good outcome. We characterized the genomic and phenotypic characteristics of papillary carcinomas to determine whether they would constitute an entity distinct from grade- and oestrogen receptor (ER)-matched invasive ductal carcinomas of no special type (IDC-NSTs). The phenotype of 63 papillary carcinomas of the breast and grade- and ER-matched IDC-NSTs was determined by immunohistochemistry. DNA of sufficient quality was extracted from 49 microdissected papillary carcinomas and 49 microdissected grade- and ER-matched IDC-NSTs. These samples were subjected to high-resolution microarray-based comparative genomic hybridization (aCGH) and Sequenom MassARRAY sequencing analysis of 19 known oncogenes. Papillary carcinomas were predominantly of low histological grade, expressed immunohistochemical markers consistent with a luminal phenotype, and a lower rate of lymph node metastasis and p53 expression than grade- and ER-matched IDC-NSTs. Papillary carcinomas displayed less genomic aberrations than grade- and ER-matched IDC-NSTs; however, the patterns of gene copy number aberrations found in papillary carcinomas were similar to those of ER- and grade-matched IDC-NSTs, including 16q losses. Furthermore, PIK3CA mutations were found in 43% and 29% of papillary carcinomas and grade- and ER-matched IDC-NSTs, respectively. The genomic profiles of encapsulated, solid and invasive papillary carcinomas, the three morphological subtypes, were remarkably similar. Our results demonstrate that papillary carcinomas are a homogeneous special histological type of breast cancer. The similarities in the genomic profiles of papillary carcinomas and grade- and ER-matched IDC-NSTs suggest that papillary carcinomas may be best positioned as part of the spectrum of ER-positive breast cancers, rather than as a distinct entity. Furthermore, the good prognosis of papillary carcinomas may stem from the low rates of lymph node metastasis and p53 expression, low number of gene copy number aberrations and high prevalence of PIK3CA mutations. Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.