These authors contributed equally to this study.
Coordinated expression of galectin-3 and caveolin-1 in thyroid cancer†
Article first published online: 10 JUL 2012
Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 228, Issue 1, pages 56–66, September 2012
How to Cite
Shankar, J., Wiseman, S. M., Meng, F., Kasaian, K., Strugnell, S., Mofid, A., Gown, A., Jones, S. J. and Nabi, I. R. (2012), Coordinated expression of galectin-3 and caveolin-1 in thyroid cancer. J. Pathol., 228: 56–66. doi: 10.1002/path.4041
No conflicts of interest were declared.
- Issue published online: 30 JUL 2012
- Article first published online: 10 JUL 2012
- Accepted manuscript online: 18 APR 2012 06:45AM EST
- Manuscript Accepted: 7 APR 2012
- Manuscript Revised: 13 MAR 2012
- Manuscript Received: 22 NOV 2011
- thyroid cancer;
- tumour cell migration;
- focal adhesions;
- tissue microarray
Galectin-3 (Gal3) is the single most accurate marker for the diagnosis of differentiated thyroid cancer (DTC). Gal3 overrides the tumour suppressor activity of caveolin-1 (Cav1) and functions in concert with Cav1 to promote focal adhesion turnover and tumour cell migration and invasion. To study their coordinated role in progression of a human cancer, we investigated the expression of Gal3 and Cav1 in specimens of human benign thyroid lesions, DTC and anaplastic thyroid cancer (ATC). Gal3 and Cav1 expression is significantly associated with DTC and ATC, but not benign nodules. Essentially all Cav1-positive DTC cancers express Gal3, supporting the synergistic activity of these two proteins in DTC progression. Similarly, coordinated elevated Gal3/Cav1 expression was observed in three DTC-derived cell lines (papillary TCP1 and KTC1 and follicular FTC133) but only one (ACT1) of five ATC-derived cell lines. Using siRNA knockdown, Gal3 and Cav1 were shown to be required for RhoA GTPase activation, stabilization of focal adhesion kinase (FAK; a measure of focal adhesion signalling and turnover) and increased migration of the DTC cell lines studied, but not the ATC cell lines, including ACT1, which expresses elevated levels of Gal3 and Cav1. Co-expression of Gal3 and Cav1 in the T238 anaplastic cell line stabilized FAK-GFP in focal adhesions. Gal3 and Cav1 therefore function synergistically to promote focal adhesion signalling, migration and progression of DTC. Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.