No conflicts of interest were declared.
Platelet-derived growth factor receptor-α promotes lymphatic metastases in papillary thyroid cancer†
Article first published online: 4 SEP 2012
Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 228, Issue 2, pages 241–250, October 2012
How to Cite
Zhang, J., Wang, P., Dykstra, M., Gelebart, P., Williams, D., Ingham, R., Adewuyi, E. E., Lai, R. and McMullen, T. (2012), Platelet-derived growth factor receptor-α promotes lymphatic metastases in papillary thyroid cancer. J. Pathol., 228: 241–250. doi: 10.1002/path.4069
- Issue published online: 10 SEP 2012
- Article first published online: 4 SEP 2012
- Accepted manuscript online: 29 JUN 2012 03:09AM EST
- Manuscript Accepted: 26 JUN 2012
- Manuscript Revised: 23 JUN 2012
- Manuscript Received: 26 NOV 2011
- platelet-derived growth factor receptor;
- papillary thyroid cancer
Lymph node metastases are common in papillary thyroid cancer (PTC) and can be resistant to surgical extirpation or radioiodine ablation. We examined the role of platelet-derived growth factor receptor (PDGFR) in mediating lymph node metastases in PTC. Clinical specimens of PTC (n = 137) were surveyed in a tissue array and by western blots to examine the relationship between expression of the α and β subunits of PDGFR and lymph node metastases. PDGFR-α was found at high levels in primary tumours with known lymphatic metastases but not in those tumours lacking nodal involvement (p < 0.0001). However, PDGFR-β expression was not linked to metastatic disease (p = 0.78) as it was found in virtually all PTC specimens. A matching analysis in fresh PTC specimens (n = 13) confirmed that PDGFR-α expression was strongly linked to metastatic spread (p = 0.0047). PDGFR-α and -β were not found in normal thyroid tissue (p < 0.0001). PTC cell lines selectively expressing PDGFR-α or -β were assessed for invasive potential and activation of downstream signal transduction pathways. PTC cell lines expressing PDGFR-α responded to PDGF-BB stimulation with increased invasive potential and this process can be blocked by the tyrosine kinase receptor inhibitor sunitinib (p < 0.009). Cell lines with only PDGFR-β, or no PDGFR, did not show significant changes in invasive potential. Activation of PDGFR-α led to downstream up-regulation of both the MAPK/ERK and PI3K/Akt pathways and disruption of either pathway is sufficient to block PDGFR-mediated increases in invasive potential. Thus, PDGFR-α is associated with lymph node metastases in papillary thyroid carcinoma and PDGFR-α promotes increased invasive potential in PTC cell lines. PDGFR-α is a strong candidate for a diagnostic biomarker to identify patients at risk of nodal metastases. Our results also strengthen the rationale for selection of tyrosine kinase receptor inhibitors that target PDGFR in the treatment of progressive, metastatic PTC. Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.