RNF43 is a tumour suppressor gene mutated in mucinous tumours of the ovary

Authors

  • Georgina L Ryland,

    1. Victorian Breast Cancer Research Consortium (VBCRC), Cancer Genetics Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
    2. Centre for Cancer Research, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
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  • Sally M Hunter,

    1. Victorian Breast Cancer Research Consortium (VBCRC), Cancer Genetics Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
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  • Maria A Doyle,

    1. Bioinformatics Core Facility, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
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  • Simone M Rowley,

    1. Victorian Breast Cancer Research Consortium (VBCRC), Cancer Genetics Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
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  • Michael Christie,

    1. Department of Anatomical Pathology, Royal Melbourne Hospital, Parkville, Victoria, Australia
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  • Prue E Allan,

    1. Department of Pathology, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
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  • David DL Bowtell,

    1. Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria, Australia
    2. Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria, Australia
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  • Australian Ovarian Cancer Study Group,,

    1. Australian Ovarian Cancer Study Group members and affiliations can be found at http://www.aocstudy.org
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  • Kylie L Gorringe,

    1. Victorian Breast Cancer Research Consortium (VBCRC), Cancer Genetics Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
    2. Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria, Australia
    3. Department of Pathology, University of Melbourne, Parkville, Victoria, Australia
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    • These authors contributed equally to this study.

  • Ian G Campbell

    Corresponding author
    1. Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria, Australia
    2. Department of Pathology, University of Melbourne, Parkville, Victoria, Australia
    • Victorian Breast Cancer Research Consortium (VBCRC), Cancer Genetics Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
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    • These authors contributed equally to this study.


  • No conflicts of interest were declared.

Correspondence to: IG Campbell, Victorian Breast Cancer Research Consortium Cancer Genetics Laboratory, Peter MacCallum Cancer Centre, Locked Bag No. 1 A'Beckett Street, Victoria 8006, Australia. E-mail: ian.campbell@petermac.org

Abstract

Mucinous carcinomas represent a distinct morphological subtype which can arise from several organ sites, including the ovary, and their genetic characteristics are largely under-described. Exome sequencing of 12 primary mucinous ovarian tumours identified RNF43 as the most frequently somatically mutated novel gene, secondary to KRAS and mutated at a frequency equal to that of TP53 and BRAF. Further screening of RNF43 in a larger cohort of ovarian tumours identified additional mutations, with a total frequency of 2/22 (9%) in mucinous ovarian borderline tumours and 6/29 (21%) in mucinous ovarian carcinomas. Seven mutations were predicted to truncate the protein and one missense mutation was predicted to be deleterious by in silico analysis. Six tumours had allelic imbalance at the RNF43 locus, with loss of the wild-type allele. The mutation spectrum strongly suggests that RNF43 is an important tumour suppressor gene in mucinous ovarian tumours, similar to its reported role in mucinous pancreatic precancerous cysts. Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

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