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path4192-sup-0001-FigureS1.docWord document192K(A) B6C3Fe a/a-Csf1op/J mice were genotyped by the TaqMan allelic discrimination method. Three populations of mice: wild-type, heterozygote, and homozygote were clearly identified. (B) Flow cytometry analysis of CD11b+CD45low microglial cells, CD11b+CD45high macrophages, and CD11bCD45+ lymphocytes showed the diminished percentage of microglia cells in naïve op/op compared with wt mice; n = 6 mice per group, *p < 0.05 (left panel). EGFP-expressing GL261 glioma cells were intracerebrally inoculated to op/op and wild-type mice. After 15 days analysis of number of infiltrating immune cells microglia and macrophages was performed. Flow cytometry analysis of CD11b+CD45low, CD11b+CD45high cells and CD11b-CD45+ lymphocytes in tumour-bearing brains shows no significant differences in percentage of microglia in op/op and wt mice; n = 6 mice per group (right panel).
path4192-sup-0002-FigureS2.docWord document206K(A) The expression of Cd68, Itgam, Ptprc, Ccr2, and Mrc1 genes was determined using qPCR in CD11b+ cells (microglia/macrophages) isolated from sham operated and EGFP-GL261glioma-bearing brains at 15 days after implantation. (B) The levels of Cd68 mRNA were determined using qPCR in CD11b+CD45low (microglia), CD11b+CD45high (macrophages) cells isolated by FACS from EGFP-GL261 glioma-bearing brains on day 15 after implantation. Monocytes (CD11b+) were magnetically isolated from blood of EGFP-GL261 glioma-bearing mice.

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