No conflicts of interest were declared.
Distinct roles of CSF family cytokines in macrophage infiltration and activation in glioma progression and injury response
Version of Record online: 7 JUN 2013
Copyright © 2013 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The Journal of Pathology
Volume 230, Issue 3, pages 310–321, July 2013
How to Cite
Sielska, M., Przanowski, P., Wylot, B., Gabrusiewicz, K., Maleszewska, M., Kijewska, M., Zawadzka, M., Kucharska, J., Vinnakota, K., Kettenmann, H., Kotulska, K., Grajkowska, W. and Kaminska, B. (2013), Distinct roles of CSF family cytokines in macrophage infiltration and activation in glioma progression and injury response. J. Pathol., 230: 310–321. doi: 10.1002/path.4192
- Issue online: 7 JUN 2013
- Version of Record online: 7 JUN 2013
- Accepted manuscript online: 20 MAR 2013 07:50AM EST
- Manuscript Accepted: 13 MAR 2013
- Manuscript Revised: 3 MAR 2013
- Manuscript Received: 13 NOV 2012
|path4192-sup-0001-FigureS1.doc||Word document||192K||(A) B6C3Fe a/a-Csf1op/J mice were genotyped by the TaqMan allelic discrimination method. Three populations of mice: wild-type, heterozygote, and homozygote were clearly identified. (B) Flow cytometry analysis of CD11b+CD45low microglial cells, CD11b+CD45high macrophages, and CD11b−CD45+ lymphocytes showed the diminished percentage of microglia cells in naïve op/op compared with wt mice; n = 6 mice per group, *p < 0.05 (left panel). EGFP-expressing GL261 glioma cells were intracerebrally inoculated to op/op and wild-type mice. After 15 days analysis of number of infiltrating immune cells microglia and macrophages was performed. Flow cytometry analysis of CD11b+CD45low, CD11b+CD45high cells and CD11b-CD45+ lymphocytes in tumour-bearing brains shows no significant differences in percentage of microglia in op/op and wt mice; n = 6 mice per group (right panel).|
|path4192-sup-0002-FigureS2.doc||Word document||206K||(A) The expression of Cd68, Itgam, Ptprc, Ccr2, and Mrc1 genes was determined using qPCR in CD11b+ cells (microglia/macrophages) isolated from sham operated and EGFP-GL261glioma-bearing brains at 15 days after implantation. (B) The levels of Cd68 mRNA were determined using qPCR in CD11b+CD45low (microglia), CD11b+CD45high (macrophages) cells isolated by FACS from EGFP-GL261 glioma-bearing brains on day 15 after implantation. Monocytes (CD11b+) were magnetically isolated from blood of EGFP-GL261 glioma-bearing mice.|
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