path4192-sup-0001-FigureS1.docWord document192K(A) B6C3Fe a/a-Csf1op/J mice were genotyped by the TaqMan allelic discrimination method. Three populations of mice: wild-type, heterozygote, and homozygote were clearly identified. (B) Flow cytometry analysis of CD11b+CD45low microglial cells, CD11b+CD45high macrophages, and CD11bCD45+ lymphocytes showed the diminished percentage of microglia cells in naïve op/op compared with wt mice; n = 6 mice per group, *p < 0.05 (left panel). EGFP-expressing GL261 glioma cells were intracerebrally inoculated to op/op and wild-type mice. After 15 days analysis of number of infiltrating immune cells microglia and macrophages was performed. Flow cytometry analysis of CD11b+CD45low, CD11b+CD45high cells and CD11b-CD45+ lymphocytes in tumour-bearing brains shows no significant differences in percentage of microglia in op/op and wt mice; n = 6 mice per group (right panel).
path4192-sup-0002-FigureS2.docWord document206K(A) The expression of Cd68, Itgam, Ptprc, Ccr2, and Mrc1 genes was determined using qPCR in CD11b+ cells (microglia/macrophages) isolated from sham operated and EGFP-GL261glioma-bearing brains at 15 days after implantation. (B) The levels of Cd68 mRNA were determined using qPCR in CD11b+CD45low (microglia), CD11b+CD45high (macrophages) cells isolated by FACS from EGFP-GL261 glioma-bearing brains on day 15 after implantation. Monocytes (CD11b+) were magnetically isolated from blood of EGFP-GL261 glioma-bearing mice.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.