Conflict of Interest: Nothing to report.
Application of flow cytometry in pediatric hematology-oncology†
Article first published online: 1 APR 2011
Copyright © 2011 Wiley-Liss, Inc.
Pediatric Blood & Cancer
Volume 57, Issue 1, pages 18–29, 15 July 2011
How to Cite
Varma, N. and Naseem, S. (2011), Application of flow cytometry in pediatric hematology-oncology. Pediatr. Blood Cancer, 57: 18–29. doi: 10.1002/pbc.22954
- Issue published online: 9 MAY 2011
- Article first published online: 1 APR 2011
- Manuscript Accepted: 9 NOV 2010
- Manuscript Received: 20 APR 2010
- acute lymphoblastic leukemia;
- acute myeloid leukemia;
- flow cytometry;
Applications of flow cytometry in pediatric cancers have expanded substantially in recent years. In acute leukemias, the commonest childhood cancer, flow cytometry can now define complex antigenic profiles that are associated with specific cytogenetic/molecular defects and can also directly identify BCR-ABL fusion protein. Flow cytometry based scoring system has been described for diagnosis of myelodysplastic syndromes. In solid tumors, flow cytometry was previously used mainly to determine DNA content for prognosis; however, recent studies in children with neuroblastoma and Ewing sarcoma have identified its diagnostic utility. In this review, we will discuss the current and future applications of flow cytometry in pediatric hematology-oncology. Pediatr Blood Cancer 2011;57:18–29. © 2011 Wiley-Liss, Inc.