Conflict of interest: Nothing to declare.
Reduced expression of transforming growth factor-β1 and correlated elevation of interleukin-17 and interferon-γ in pediatric patients with chronic primary immune thrombocytopenia (ITP)†
Article first published online: 30 JUN 2011
Copyright © 2011 Wiley-Liss, Inc.
Pediatric Blood & Cancer
Volume 57, Issue 4, pages 636–640, October 2011
How to Cite
Wang, J.-D., Chang, T.-K., Lin, H.-K., Huang, F.-L., Wang, C.-J. and Lee, H.-J. (2011), Reduced expression of transforming growth factor-β1 and correlated elevation of interleukin-17 and interferon-γ in pediatric patients with chronic primary immune thrombocytopenia (ITP). Pediatr. Blood Cancer, 57: 636–640. doi: 10.1002/pbc.22984
- Issue published online: 8 AUG 2011
- Article first published online: 30 JUN 2011
- Manuscript Accepted: 23 NOV 2010
- Manuscript Received: 1 SEP 2010
- Taichung Veterans General Hospital of Taiwan. Grant Numbers: TCVGH 986501A, TCVGH 986504B, TCVGH 996505B
- chronic primary immune thrombocytopenia;
- T helper cells
Dysregulated T helper (Th) cells are considered important in the pathophysiology of chronic primary immune thrombocytopenia (ITP). The present study investigated whether levels of Th cytokines in pediatric patients with chronic ITP were different compared with healthy controls.
Fifty-seven pediatric patients with chronic ITP and 28 healthy controls were enrolled. Patients were divided into three groups based on their platelet counts at the time of the study: (i) active disease <50 × 109/l (n = 23), (ii) stable disease 50–150 × 109/l (n = 23), and (iii) in remission >150 × 109/l (n = 11). Plasma concentration of Th1 [interferon gamma (INF-γ), interleukin 2 (IL-2)], Th2 (IL-4, IL-10), Th3 [transforming growth factor-β1 (TGF-β1)], and Th17 (IL-17) cytokines were investigated by enzyme-linked immunosorbent assay.
IFN-γ was increased in patients with active (P < 0.001) and stable disease (P = 0.026) when compared with controls. The IL-17 level was significantly higher in all of the 3 patient groups. In addition, there was a positive correlation between IL-17 and IFN-γ levels in chronic ITP patients (r = 0.640, P < 0.001). Reduced TGF-β1 expression was observed in patients with active (P < 0.001) and stable disease (P = 0.001) in comparison with controls. Moreover, TGF-β1 level in patients was positively correlated with the platelet count (r = 0.355, P = 0.007).
Elevation of IL-17 and IFN-γ may be an important dysregulation of cellular immunity in pediatric patients with chronic ITP. The disease activity is associated with reduced production of TGF-β1. Pediatr Blood Cancer 2011; 57: 636–640. © 2011 Wiley-Liss, Inc.