Systematic review and meta-analysis of randomized trials of central nervous system directed therapy for childhood acute lymphoblastic leukemia

Authors


  • Conflicts of interest: Nothing to declare.

Abstract

Treatment of the central nervous system (CNS) is an essential therapy component for childhood acute lymphoblastic leukemia (ALL). Individual patient data from 47 trials addressing 16 CNS treatment comparisons were analyzed. Event-free survival (EFS) was similar for radiotherapy versus intrathecal (IT), and radiotherapy plus IT versus IV methotrexate (IV MTX) plus IT. Triple intrathecal therapy (TIT) gave similar EFS but poorer survival than intrathecal methotrexate (IT MTX), but additional IV MTX improved both outcomes. One trial resulted in similar EFS and survival with IV MTX plus IT MTX versus TIT alone. Radiotherapy can generally be replaced by IT therapy. TIT should be used with effective systemic therapy such as IV MTX. Pediatr Blood Cancer 2013;60:185–195. © 2012 Wiley Periodicals, Inc.

INTRODUCTION

Pre-symptomatic treatment of the central nervous system (CNS) is an indispensable component of the successful therapy of childhood acute lymphoblastic leukemia (ALL) 1. Historically, pre-symptomatic cranial or craniospinal irradiation was a standard component of treatment for all patients. As concerns about the long-term consequences of radiation increased 2, irradiation was first limited to the brain, then decreased in dose, and finally largely replaced by effective intrathecal and systemic chemotherapy, but retained for patients with high-risk disease or leukemic blasts identifiable in the cerebrospinal fluid at diagnosis. In some contemporary clinical trials, irradiation has been omitted for all patients 3, 4. To successfully prevent the progression or recurrence of leukemia in the CNS, optimal systemic and intrathecal therapy is necessary 1. In 2003, we performed a collaborative meta-analysis of randomized trials addressing issues of pre-symptomatic CNS therapy that initiated accrual prior to 1994 5. In this study, we update this work, and include additional trials that commenced before 2000 to allow adequate follow-up time.

METHODS

CNS-directed interventions include cranial or craniospinal irradiation, intrathecal drugs, intravenous methotrexate at a dose of ≥0.5 g/m2 plus leucovorin, or intravenous mercaptopurine. This study focuses on newly diagnosed patients and excludes those who received craniospinal irradiation, a strategy no longer in use. Intravenous mercaptopurine will be reported separately.

Other drugs, which also have an effect on CNS control, in particular type of steroid 6–8 will be addressed in a future study, but a summary of steroid use in the current set of trials has been recorded. Thioguanine also appears to influence CNS control, but is not currently in use in most protocols because of concerns about toxicity 9.

Trials were identified following detailed search of electronic databases including MEDLINE and EMBASE. Additional hand-searching of review articles, meeting abstracts, reference lists of published trials, and the content lists of major cancer and general medical journals was undertaken. Members of the Childhood ALL Collaborative Group and other experts were consulted to ensure the completeness of the resulting list of trials. For all trials, ethical committee review and individual/family informed consent were obtained as per national standard. Data collected on each trial included period of recruitment, eligibility criteria, randomized treatment elements and timing, and any CNS-directed treatment given in addition to the randomized question.

For all trials the following information was sought for each patient aged 21 years or younger at random assignment to treatment: date of diagnosis, age or date of birth, gender, white blood count (WBC) at diagnosis, immunophenotype, date of random assignment to treatment and treatment allocation, site of any first relapse, as well as time of first remission, relapse, death, or last contact. In addition, the date and type of any secondary tumor were sought. Data were checked for internal consistency, balance between treatment groups by initial features, randomization dates, and length of follow-up, and consistency with published results.

All first event outcomes were measured from date of randomization. Primary outcomes were event-free survival (EFS) and overall survival (OS). Any induction failure, relapse or death was counted as an adverse event. As many trials did not record secondary tumors, these events were not included in analyses. Secondary outcome measures were no remission (defined as death without remission achievement), isolated CNS relapse, any CNS relapse (isolated CNS relapse plus combined relapses with CNS involvement), non-CNS relapse, and death in remission, which included death due to secondary tumor. Relapse data were obtained only for first relapse. Survival from relapse was also analyzed.

Standard statistical meta-analysis methods were used. Within trials, analyses examined time from randomization to any event, with the observed minus expected (o − e) number of events and its variance (v) obtained by the log-rank method. These o − e values were then added over all trials to produce a total (T), with variance (V) equal to the sum of the separate variances. These were used to calculate an overall odds ratio (OR), or ratio of event rates, and its 95% confidence interval (CI) equal to exp(T/V ± 1.96/√V). Results are presented as forest plots with a square representing the point estimate of the OR and a horizontal line showing the 99% CI for each trial. The size of the square is proportional to the number of events, not patients. Overall estimates are shown by a diamond with the width representing the 95% CI. All P values given are two-sided. Heterogeneity between the effects in different trials or subgroups was tested with equation image equal to S − T2/V, where S is the sum of (o − e)2/v from each of n trials or n subgroups 10.

T and V obtained by summing o − e and v from log rank analyses restricted to each one year time period were used to estimate the log OR, b, for each year. The estimated overall event rate in each time period, r, equals the number of events divided by the number of person years, and the probability of surviving event-free during that year is P = exp(−r). Descriptive survival curves were drawn from the separate probability estimates P + 0.5p(p − 1)b for one treatment group, and p − 0.5p(p − 1)b for the other treatment group 10.

Trials were categorized a priori and the results combined according to the treatment comparisons and background CNS-directed treatments. We examined conventional pre-specified subgroups by gender, age group (<10 and ≥10 years), WBC (<10, 10–19, 20–49, 50–99, and ≥100 × 109/L), and immunophenotype (B-lineage and T-lineage).

RESULTS

Data were available for 47 trials, ten of which were not included in the previous analyses. Forty-five trials had a median follow-up of at least 5 years. Studies were grouped into 11 categories by randomized comparison. Table I gives details of the CNS-directed treatments 11–56. Steroid use is given in supplementary Table S1. Supplementary Tables S2 and S3 show the distribution of patient and disease characteristics and median follow-up for each trial.

Table I. Summary of CNS-Directed Treatments
TrialYearReferencesCNS-directed therapy
  • AraC, cytosine arabinoside; CRT, cranial irradiation; CSCRT, craniospinal irradiation; DITa, IT MTX + IT AraC; DITb, IT MTX + IT Hc; DITc, IT MTX + IT P; Dx, dexamethasone; f, female; Hc, hydrocortisone; IT, intrathecal; m, male; mp, mercaptopurine; MTX, methotrexate; P, prednisone; R, randomization; TITa, IT MTX + IT AraC + IT Hc; TITb, IT MTX + IT AraC + IT Dx; TITc, IT MTX + IT AraC + IT P.

  • a

    Not in previous paper;

  • b

    Data not available.

A: TIT vs. IT therapy
 CCG-1952a1996

11

DITa × 1 + IT MTX × 1 R: TITa × 14 (f) or 18 (m) vs. IT MTX × 14 (f) or 18 (m)
B: Radiotherapy plus IT therapy vs. extra IT therapy
 a: Radiotherapy vs. IT therapy
  CCG-1611978

12

IT MTX × 6 R: 18 Gy CRT vs. IT MTX × 8
  CCG-1051983

13

IT MTX × 6 R: 18 Gy CRT vs. IT MTX × 8 (f) or 14 (m)
  INEN-P831983 IT MTX × 5 R: 18 Gy CRT vs. IT MTX × 12
  CCG-18821989

14

IT MTX × 14 (f) or 18 (m) + IT AraC × 1 R: 18 Gy CRT vs. IT MTX × 7
 b: Radiotherapy (+ IT) vs. DIT or TIT therapy
  SWOG 7623/AlinC12b1976

15

R: 24 Gy CRT + IT MTX × 5 vs. TITa × 22
  LAL 7/781978

16

R: 24 Gy CRT + IT MTX × 6 vs. DITa × 10
  INS 841984

17

TITa × 6 R: 18 Gy CRT vs. TITa × 12
  INEN-P851985 R: 18 Gy CRT + IT MTX × 5 vs. TITa × 17
 c: Radiotherapy + DIT vs. TIT therapy
  DFCI ALL 95-001a1996

18

IT AraC × 1 + TITa × 2 R: 18Gy CRT + DITa × 6 vs. TITa × 12
C: Addition of IV methotrexate to long-term IT therapy or radiotherapy with IT therapy
 a: TIT therapy ±  IV methotrexate
  SJCRH Total XIIIA1991

19

TITa × 13 or 17 + 2 g/m2IV MTX × 9 or 10 R: ±1 g/m2 IV MTX × 1
  POG 9005a1991

20

1 g/m2 IV mp × 17–19 + TITA × 16 R: ±1 g/m2 IV MTX × 12
  FRALLE 93 LR1993

21

TITc × 16 R: ±1.5 g/m2 IV MTX × 6
  FRALLE 93 IR1993

21

TITc × 18 R: ±8 g/m2 IV MTX × 4
  SJCRH Total XIIIBa1994

22

1 g/m2 IV mp + TITa × 13 or 15 + 2 g/m2 IV MTX × 10 R: ±1 g/m2 IV MTX × 1
 b: IT therapy ± IV methotrexate
  CCG-1391984

23

IT MTX × 15 (f) or 20 (m) R: ±0.5 g/m2 IV MTX × 24 (f) or 33 (m)
  DFCI 870011987

24

IT AraC × 1 + IT MTX × 10 (HR: + 18 Gy CRT) R: ±4 g/m2 IV MTX × 1
  UKALLXI LWCC1990

25

IT MTX × 16 R: ±6–8 g/m2 IV MTX × 3
 c: Radiotherapy with IT therapy ± IV methotrexate
  DFCI 810011981

26

18 or 28 Gy CSCRT + IT AraC × 1 + IT MTX × 8 R: ±4 g/m2IV MTX × 1
  POG 9404a1994

27

TITa × 11 + 18 Gy CRT R: ±5 g/m2 IV MTX × 4
D: IV methotrexate + IT therapy vs. TIT
  POG8035/8036a1981

28

TITa × 6 R: 1 g/m2 IV MTX × 17 + IT MTX × 4 vs. TITa × 17
E: Addition of IT therapy to radiotherapy plus short-term IT therapy
  CALGB-7113b1971

29

24 Gy CRT + IT MTX × 12 R: ±IT MTX × 3
  CCG-1621978

30

18 Gy CRT + IT MTX × 6 R: ±IT MTX × 8
  UKALLVII1979

31

18 or 24 Gy CRT + IT MTX × 5 R: ±IT MTX × 8
F: Radiotherapy plus IT therapy vs. IV methotrexate plus IT therapy
  CLB 76111976

32

IT MTX × 6 R: 24 Gy CRT vs. 0.5 g/m2 IV MTX × 3
  NCI 77-02b1980

33

R: 18–24 Gy CRT + IT MTX × 5 vs. 33.6 g/m2 IV MTX × 10
  ALL-BFM-811981

34

IT MTX × 6 R: 12–18 Gy CRT vs. 0.5 g/m2 IV MTX × 4
  Jena ALL VII 811981

35

IT MTX × 8 R: 18 Gy CRT vs. 0.5 g/m2 IV MTX × 4
  JCCLSG L-8741987

36

DITb × 3 R: 18 Gy CRT vs. 2 g/m2 IV MTX × 3 + DITb × 6
  JCCLSG I-8741987

36

(2 g/m2 × 1 + 4.5 g/m2 × 20) IV MTX + DITb × 1 R: 18 Gy CRT + DITb × 2 vs. 4.5 g/m2 IV MTX × 3
  GCMTLA1988

37

TITb × 6 R: 12–18 Gy CRT vs. 0.5 g/m2 IV MTX × 4 + TITb × 6
  TCCSG L89-12b1989

38

TITa × 4 + DITa × 3 R: 18 Gy CRT vs. 3 g/m2 IV MTX
  UKALLXI HWCC1990

25

IT MTX × 7 or 9 R: 24 Gy CRT vs. 6–8 g/m2 IV MTX × 3 + IT MTX × 8 or 9
  TCCSG L92-13 HRb1992

38

DITb × 4 + TITa × 3 R: 18 Gy CRT + DITb × 1 vs. 3 g/m2 IV MTX × 2
  MRC ALL97a1997

39

IT MTX × 7 or 9 R: 24 Gy CRT vs. 6–8 g/m2 IV MTX × 3 + IT MTX × 8 or 9
G: Higher dose of IV methotrexate
  POG 9405a1994

40

TITa × 22 R: 2.5 g/m2 IV MTX × 12 vs. 1 g/m2 IV MTX × 12
  POG 9406a1994

40

TITa × 18 + 1 g/m2 IV mp × 6 R: 2.5 g/m2 IV MTX × 6 vs. 1 g/m2 IV MTX × 6
H: Higher doses of radiotherapy
  UKALL V1976

41

IT MTX × 5 R: 24 Gy vs. 21 Gy CRT
  UKALLVI(i)1978

42

IT MTX × 8 or IT MTX × 6 + IT AraC × 2 + 0.5 g/m2 IV MTX × 3 R: 24 Gy vs. 21 Gy CRT
  UKALLVI(ii)1978

42

IT MTX × 8 or IT MTX × 6 + IT AraC × 2 + 0.5 g/m2 IV MTX × 3 R: 24 Gy vs. 18 Gy CRT
  UKALLVII1979

31

IT MTX × 5 R: 24 Gy vs. 18 Gy CRT
  GBTLI-801980

43

IT MTX × 13 R: 24 Gy vs. 18 Gy CRT
  TCCSG L81-101981

44

DITa × 5 R: 24 Gy vs. 18 Gy CRT
  ALL-BFM-831983

34

IT MTX × 8 + 0.5 g/m2 IV MTX × 4 R: 18 Gy vs. 12 Gy CRT
I: Addition of radiotherapy
 a: Addition to IT
  POG CNS2b1970

45

IT MTX × 20 R: ±24 Gy CRT
  CLB 7111b1971

46

IT MTX × 6 R: ±24 Gy CRT
  SWOG 690/691/Alinc9b1971

47

TITa × 10 R: ±18–24 Gy CRT
  DFCI-SFCCb1972

48

IT MTX × 9 R: ±24 Gy CRT
  CLB 7411b1974

49

IT MTX × 6 R: ±24 Gy CRT
  CCG-1011974

50

IT MTX × 6 R: ±24 Gy CRT
  CCG-123a1983

51

IT AraC × 1 + IT MTX × 6 R: ±18Gy CRT
 b: Addition to IV with IT
  EORTC 588321983

52

2.5 g/m2 IV MTX × 4 + IT MTX × 7 R: ±16–20 Gy CRT
J: Higher dose of IV methotrexate vs. more IT or DIT therapy
  FRALLE 871987

53

DITc × 5 R: 8 g/m2 IV MTX × 4 vs. 3 g/m2 IV MTX × 4 + DITc × 5
  FRALLE 891989

21

IT MTX × 5 R: 8 g/m2IV MTX × 4 vs. 3 g/m2IV MTX × 4 + IT MTX × 5
K: Addition of IV methotrexate plus IT, DIT, or TIT therapy to radiotherapy plus IT or TIT therapy and/or IV methotrexate
  Jena ALLVII 811981

35

12 or 18 Gy CRT + IT MTX × 8 R: ±0.5 g/m2 IV MTX × 4 + IT MTX × 4
  TCCSG L84-11 SR1984

38

18 Gy CRT + TITa × 5 + 0.5 g/m2 IV MTX × 4 + IT MTX × 4 R: ±0.5 g/m2 IV MTX × 3 + DITb × 6
  TCCSG L84-11 HR1984

38

24 Gy CRT + TITa × 5 + 0.5g/m2 IV MTX × 12 +DITb × 12 R: ±0.5 g/m2 IV MTX × 3 + TITa × 6
Other comparisons without data
  ALGB 6801b1968

54

R: IT MTX × 15
  GATLA-70b1970

55

R: ±24 Gy CRT + IT MTX × 5
  NCI 72-1b1971

56

24 Gy CRT R: IT AraC × 38 vs. IT MTX × 35
  NCI-84-C-153Ab1984 R: 33.6 g/m2 IV MTX × 10 vs. IT MTX × 8

Grouping of Trials

A: Triple intrathecal therapy (TIT) with methotrexate, hydrocortisone and cytarabine versus intrathecal (IT) methotrexate (IT MTX) therapy. One new trial, involving 2,029 NCI standard- risk (age <10 years and WBC <50 × 109/L) patients, addressed this question.

B: Radiotherapy plus IT therapy versus extra IT therapy. Nine trials, including one new trial, addressed this question. Data were available for eight of these trials, involving 2,995 patients, which compared treatment regimens with either 18 or 24 Gy cranial irradiation (CRT) plus some IT treatment with the same regimen without CRT but with additional doses of IT MTX or TIT, the use of double IT (DIT) in place of IT MTX, or TIT in place of double IT.

In the light of the results of the comparison of TIT versus IT MTX therapy, this group of trials was split post hoc into three subgroups: (a) CRT + IT versus extra IT, (b) CRT + IT MTX versus DIT or TIT, and (c) CRT + DIT versus TIT. We found heterogeneity among subgroups for CNS relapse (P = 0.01).

C: Addition of IV methotrexate (IV MTX) (doses500mg/m2) to long-term IT therapy or radiotherapy with IT therapy. Ten trials with 4,140 patients addressed this question, including two new trials. One trial (CCG-163d) previously included was discovered to be confounded, with other chemotherapy differences between the arms with and without IV MTX, and so it has now been excluded in the analysis. One trial (POG9005), which was previously in the “other comparisons without data” category, is now included because most protocols specified some additional low dose oral MTX in the control arm and this is considered not an important confounding factor.

Again, in the light of the IT MTX versus TIT results, this comparison of the addition of IV MTX was split into three groups according to the background treatment: (a) TIT therapy, sometimes plus IV mercaptopurine (MP) and/or some IV MTX, (b) IT MTX, and (c) CRT plus IT MTX or TIT therapy. Heterogeneity of effect between subgroups was seen for EFS (P = 0.01). Excluding group c, heterogeneity was seen between groups a and b for both CNS (P = 0.06) and non-CNS relapse (P = 0.008).

D: IV MTX plus IT MTX therapy versus triple IT therapy. Data were newly available for one trial, previously included in the “other comparison” group, involving 1,159 patients.

E: Addition of IT therapy to treatment including radiotherapy and short-term IT therapy. Data were available for 1,174 patients in two trials out of the four addressing this, previously included in the “other comparisons group”.

F: Radiotherapy plus IT therapy versus IV MTX plus IT therapy. Trials comparing CRT with IV MTX were grouped together on the assumption that small differences in IT therapy do not have a major impact. Data for eight trials (including one new trial), involving 1,635 patients, were available.

G: Higher dose of IV MTX. Two new trials, both including TIT therapy for all patients and totally involving 1,071 patients, addressed this treatment.

H: Higher doses of radiotherapy. Data were available for all eight trials, including one previously without data (GBTLI-80), involving 905 patients. Comparisons were of 24 Gy versus 21 or 18 Gy, except for one trial comparing 18 Gy versus 12 Gy.

I: Addition of radiotherapy. Data, involving 664 patients, were available for three of the eight trials addressing this question, including one new trial (CCG-123). These were previously included in the “other comparison” group. Significant heterogeneity was found for the effect on CNS relapse between (a) CCG trials, which used only IT therapy, and (b) the EORTC 58,832 trial, which included IV MTX in both arms (P = 0.0003). Therefore this comparison was split.

J: Higher dose of IV MTX versus more IT therapy. Two trials, involving 700 patients, previously included in “other comparisons” are grouped here. One used double IT and the other IT MTX.

K: Addition of IV MTX plus IT therapy to radiotherapy plus IT therapy and/or IV MTX. Three trials involving 511 patients addressed this.

Outcomes

Except as stated, treatment effects were similar in different gender, age, WBC, and immunophenotype subgroups. Figure 1A (groups A, Ba, Bb, Ca, and Cb), B (groups Cc, D, E, F, and G), and C (groups H, Ia, Ib, J, and K) show the treatment effects on EFS for each trial, and for each comparison group. Table S4 shows outcome for all event types according to treatment and results are summarized in Table II. Only 12 trials reported secondary tumor data, which, if included, would have added 21 events.

Figure 1.

Effects of treatments on event free survival. Ratios of annual event rates with each trial result represented by a square, with larger squares indicating more information, and the overall result for each comparison represented by a diamond.

Table II. Summary Results
TrialBackground CNS treatmentArm 1Arm 2aConclusions
  • CRT, cranial irradiation; CSCRT, craniospinal irradiation; DIT, double intrathecal; f, female; IT, intrathecal; IV, intravenous methotrexate; IV mp, intravenous mercaptopurine; m, male; TIT, triple intrathecal.

  • a

    Control indicates that no CNS treatment was given additional to the background.

A: TIT vs. IT therapy (n = 2,029)

 CCG-1952 11

DIT × 1 + IT × 1TIT × 14 (f) or 18 (m)IT × 14 (f) or 18 (m)TIT reduced CNS relapse compared with IT but an increase in non-CNS relapse resulted in similar EFS and worse OS (OR = 1.50; P = 0.01)
Ba: Radiotherapy plus short-term IT therapy vs. extra IT therapy (n = 2,614)

 CCG-161 12

IT × 6CRTIT × 8Adding CRT to short-term IT reduced CNS relapses (OR = 0.68; P = 0.01) compared with adding more IT doses, but did not substantially affect EFS (OR = 0.92; 95% CI: 0.80–1.05; P = 0.2)

 CCG-105 13

IT × 6CRTIT × 8 (f) or 14 (m) 
 INEN-P83IT × 5CRTIT × 12 

 CCG-1882 14

IT × 15 (f) or 19 (m)CRTIT × 7 
Bb: Radiotherapy plus short-term IT therapy vs. DIT or TIT (n = 234)

 LAL 7/78 16

 CRT + IT × 6DIT × 10Small numbers make results uncertain but OS was worse with CRT (OR = 1.63; P = 0.02)

 INS 84 17

TIT × 6CRTTIT × 12 
 INEN-P85 CRT + IT × 5TIT × 17 
Bc: Radiotherapy plus DIT vs. extra TIT (n = 155)   Insufficient data to draw any conclusions

 DFCI ALL 95-001 18

IT × 1 + TIT × 2CRT + DIT × 6TIT × 12 
Ca: Addition of IV methotrexate to TIT or TIT with IV methotrexate (n = 1,751)

 SJCRH Total XIIIA 19

TIT × 13 or 17 + IV × 9 or 10IV × 1ControlAdding IV MTX to TIT reduced both CNS (OR = 0.48; P = 0.0009) and non-CNS relapse (OR = 0.65; P = 0.002), giving better EFS (OR = 0.65; P = 0.00005) and OS (OR = 0.71; P = 0.02)

 POG 9005 20

IV mp × 17–19 + TIT × 16IV × 12Control 

 FRALLE 93 LR 21

TIT × 16IV × 6Control 

 FRALLE 93 IR 21

TIT × 18IV × 4Control 

 SJCRH Total XIIIB 22

IV mp + TIT × 13 or 15 + IV × 10IV × 1Control 
Cb: Addition of IV methotrexate to long-term IT therapy (n = 2,014)

 CCG-139 23

IT × 15 (f) or 20 (m)IV × 24 (f) or 33 (m)ControlAdding IV MTX to long-term IT probably reduced CNS relapse (OR = 0.78; P = 0.06), but did not improve EFS or OS

 DFCI 87001 24

IT × 11 (HR: + CRT)IV × 1Control 

 UKALLXI LWCC 25

IT × 16IV × 3Control 
Cc: Addition of IV methotrexate to radiotherapy with TIT or IT therapy (n = 375)

 DFCI 81001 26

CSCRT + IT × 9IV × 1ControlAdding IV MTX to CRT with short-term IT or TIT reduced CNS relapses (OR = 0.39; P = 0.008) and improved EFS (OR = 0.64; P = 0.03), but small numbers make these results less reliable

 POG 9404 27

TIT × 11 + CRTIV × 4Control 
D: IV methotrexate + IT therapy + TIT vs. extra TIT (n = 1,159)

 POG8035/8036 28

TIT × 6IV × 17 + IT × 4TIT × 17IV MTX was less effective than extra TIT in preventing CNS relapse (OR = 1.64; P = 0.01) but the reduction in non-CNS relapse (OR = 0.82; P = 0.08) resulted in equivalent EFS and OS.
E: Addition of IT therapy to radiotherapy plus short-term IT therapy (n = 1,174)

 CCG-162 30

CRT + IT × 6IT × 8ControlAdding extra IT therapy to CRT with short-term IT did not give benefit

 UKALLVII 31

CRT + IT × 5IT × 8Control 
F: Radiotherapy vs. IV methotrexate: short-term IT, DIT in both arms (IV MTX in one trial) and some additional IT or TIT in the IV MTX arm (n = 1,635)

 CLB 7,611 32

IT × 6CRTIV × 3CRT given with short-term IT, DIT or TIT, reduced CNS relapse (OR = 0.43; P < 0.00001) compared with giving IV MTX, with or without extra IT, but non-CNS relapses were increased (OR = 1.54; P = 0.00001), resulting in no difference in EFS or OS

 ALL-BFM-81 34

IT × 6CRTIV × 4 

 Jena ALL VII 81 35

IT × 8CRTIV × 4 

 JCCLSG L-874 36

DIT × 3CRTIV × 3 + TIT × 6 

 JCCLSG I-874 36

IV × 21+ DIT × 1CRT + DIT × 2IV × 3 

 GCMTLA 37

TIT × 6CRTIV × 4 + TIT × 6 

 UKALLXI HWCC 25

IT × 7 or 9CRTIV × 3 + IT × 8 or 9 

 MRC ALL97 39

IT × 7 or 9CRTIV × 3 + IT × 8 or 9 
G: Higher dose of IV methotrexate (n = 1,071)

 POG 9405 40

TIT × 222.5 g/m2 IV × 121 g/m2 IV × 12No benefit from higher dose of IV MTX

 POG 9406 40

TIT × 18 + 1 g/m2 IV mp × 62.5 g/m2 IV × 61 g/m2 IV × 6 
H: Higher doses of radiotherapy (n = 905)

 UKALL V 41

IT × 524 Gy CRT21 Gy CRTNo benefit from higher doses of CRT

 UKALLVI(i) 42

IT × 10 or IT × 8 + IV × 324 Gy CRT21 Gy CRT 

 UKALLVI(ii) 42

IT × 10 or IT × 8 + IV × 324 Gy CRT18 Gy CRT 

 UKALLVII 31

IT × 524 Gy CRT18 Gy CRT 

 GBTLI-80 43

IT × 1324 Gy CRT18 Gy CRT 

 TCCSG L81-10 44

DIT × 524 Gy CRT18 Gy CRT 

 ALL-BFM-83 34

IT × 8 + IV × 418 Gy CRT12 Gy CRT 
Ia: Addition of radiotherapy to short-term IT therapy (n = 475)

 CCG-101 50

IT × 6CRTControlAdding CRT to 6 or 7 doses of IT reduced CNS relapses (OR = 0.28; P < 0.00001) and improved EFS (OR = 0.68; P = 0.003)

 CCG-123 51

IT × 7CRTControl 
Ib: Addition of radiotherapy to IV methotrexate plus IT therapy (n = 189)

 EORTC 58,832 52

IV × 4 + IT × 7CRTControlInsufficient data to draw any conclusions
J: Higher dose of IV methotrexate vs. more IT or DIT therapy (n = 700)

 FRALLE 87 53

DIT × 58 g/m2 IV × 43 g/m2 IV × 4 + DIT × 5There was no evidence of a difference between high dose and a lower dose with extra IT or DIT

 FRALLE 89 21

IT × 58 g/m2 IV × 43 g/m2 IV × 4 + IT × 5 
K: Addition of IV methotrexate plus IT, DIT or TIT therapy to radiotherapy plus IT or TIT therapy and/or IV methotrexate (n = 511)

 Jena ALLVII 81 35

CRT + IT × 8IV × 4 + IT × 4ControlThere was no evidence of a benefit from adding

 TCCSG L84-11 SR 38

CRT + TIT × 5 + IV × 4 + IT × 4IV × 3 + DIT × 6ControlIV MTX with extra IT, DIT or TIT to a treatment which included CRT

 TCCSG L84-11 HR 38

CRT + TIT × 5 + IV × 12 + DIT × 12IV × 3 + TIT × 6Control 

Radiotherapy

Early trials, mostly of 24 Gy versus 18 or 21 Gy, indicated no evidence that higher dose CRT offers any advantage (comparison H). No significant differences were found for any endpoints (Tables II, S4, and Fig. 1C).

Where only short-term IT was used (i.e., 6 or 7 doses; Comparison Ia), CRT significantly reduced the CNS relapse rate (OR = 0.28; 95% CI: 0.18–0.43; P < 0.00001; Tables II and S4). The non-CNS relapse rates were similar and hence the overall event rate was reduced (P = 0.003), resulting in a long-term EFS benefit (Fig. 1C). There was evidence of possible heterogeneity (P = 0.02) between those aged <10 and ≥10 years, with the larger effect in younger patients where a larger proportion of the relapses involved the CNS. Survival from relapse was poorer for those relapsing after CRT so that OS was not significantly improved.

Replacing CRT with additional IT doses (Comparison Ba) showed that CRT reduced CNS relapse (OR = 0.68; 95% CI = 0.51–0.92; P = 0.01) with no difference in non-CNS relapse (Tables II and S4). As CNS relapse was a relatively rare event in these trials, no significant EFS difference emerges (Fig. 1A). Survival from CNS relapse was non-significantly worse following CRT (P = 0.08), while there was no difference in survival from non-CNS relapse, resulting in no difference in OS (Table S4).

Alternatively, in trials replacing CRT with IV MTX (Comparison F), CRT reduced CNS relapse (OR = 0.43; 95% CI: 0.34–0.56; P < 0.0001) but was less effective than IV MTX for non-CNS relapse (OR = 1.54; 95% CI 1.27–1.86; P = 0.00001; Tables II and S4). This resulted in similar overall EFS rates (OR = 0.95; 95% CI: 0.81–1.09, Fig. 1B). Survival from relapse was non-significantly worse for CNS relapse after CRT compared with after IV MTX, but better for non-CNS relapse, and there was no difference in OS.

Comparisons of CRT plus IT with DIT or TIT (Comparison Bb) or CRT plus DIT with TIT (Comparison Bc) included only small numbers (n = 225, 156, respectively). In the first group, CRT appeared to result in poorer OS (OR = 1.53; 95% CI: 1.01–2.30; P = 0.04; Tables II and S4) but no significant differences were found for any other endpoint. In the second group, CRT plus DIT yielded better CNS control (OR = 0.13; 95% CI: 0.02–0.93) but there was no difference in EFS or OS (Tables II and S4) and this is based on only one trial including 156 patients and four CNS relapses. When IV MTX was given, no benefit was seen with CRT (Comparison Ib), but fewer than 200 patients were randomized (Tables II and S4).

Combining Additional CNS Therapy With CRT

If CRT was included in treatment, along with some IT therapy, EFS rates were not improved with addition of further IT doses (OR = 0.93; 95% CI: 0.77–1.12) (Comparison E, Tables II, S4, and Fig. 1B). No significant differences were found for any endpoints (Table S4).

No significant differences were found for any endpoints in the comparison of CRT plus IT therapy and/or IV MTX with IV MTX plus IT therapy, although numbers are limited (Comparison K, Tables I, and S4).

Two trials looked at the addition of IV MTX to therapy including CRT, one trial with craniospinal irradiation plus IT MTX therapy and the other with CRT plus TIT (Comparison Cc). Although only 375 patients were randomized there was a reduction in CNS relapse (OR = 0.39; 95% CI: 0.20–0.79), EFS benefit (OR = 0.64; 95% CI: 0.43–0.95; P = 0.03), and possibly improved OS (P = 0.09; Tables II, S4, and Fig. 1B). The majority of patients in this comparison were T lineage ALL.

Treatment Without CRT

The one large trial of TIT versus IT MTX (Comparison A) showed that CNS relapses were reduced by one third with TIT (OR = 0.66; 95% CI: 0.47–0.93; P = 0.02), but non-CNS relapses were increased by three quarters (OR = 1.73; 95% CI: 1.30–2.29; P = 0.0002; Tables II and S4), and there was no difference in EFS (P = 0.3; Fig. 1A). TIT resulted in worse OS (OR = 1.50; 95% CI: 1.09–2.06; P = 0.01; 2.8% absolute difference; Fig. 2), due partly to worse post-relapse survival for non-CNS as opposed to CNS relapse with either treatment, but also to poorer survival for each relapse type after relapse on TIT (OR = 1.84; 95% CI: 1.01–3.35; P = 0.05 after CNS, OR = 1.30; 95% CI: 0.83–2.05; P = 0.3 after non-CNS relapse).

Figure 2.

Descriptive event free and overall survival curves for TIT versus IT therapy.

Adding IV MTX to TIT (Comparison Ca) reduced the overall event rate by a third (OR = 0.65; 95% CI: 0.53–0.80; P = 0.00005; Fig. 1A) due to a reduction in both CNS and non-CNS relapses (Tables II and S4), and resulting in absolute difference of 8.2% in EFS and 3.6% in OS (Fig. 3). Where all patients received IT MTX therapy (Comparison Cb), there was no apparent benefit for IV MTX (EFS: OR = 0.93; 95% CI: 0.80–1.08; P = 0.3; Figs. 1A and 4), although the rate of CNS relapses may be somewhat lower (OR = 0.78; 95% CI: 0.60–1.01; P = 0.06; Tables II and S4). No differences were found for survival from relapse or OS in either of these comparisons.

Figure 3.

Descriptive event free and overall survival curves for the addition of IV methotrexate to TIT.

Figure 4.

Descriptive event free and overall survival curves for the addition of IV methotrexate to IT therapy.

In one large trial comparing IV MTX plus IT therapy with TIT alone (Comparison D), CNS relapse was higher with IV MTX plus IT MTX (OR = 1.64; 95% CI: 1.13–2.39; P = 0.01; Tables II and S4) but the lower non-CNS relapse rate, albeit not significant, resulted in similar EFS (OR = 0.98; 95% CI: 0.82–1.17, Fig. 1B) and OS.

In the comparison of higher dose IV MTX (i.e., 2.5 g/m2 vs. 1 g/m2; Comparison G), no significant differences were found for any endpoint, including EFS (OR = 0.93; 95% CI: 0.74–1.16; Tables II, S4, and Fig. 1B).

Similarly, comparisons of high dose IV MTX (8 g/m2) with lower dose (3 g/m2) plus IT MTX or DIT (Comparison J) showed no significant differences for any endpoint (Tables II and S4).

DISCUSSION

This study demonstrated that effective systemic and IT chemotherapy can yield EFS at least comparable to that of CRT with or without additional IT therapy; the findings extend those of the previous meta-analysis showing that CRT could generally be replaced by long-term IT therapy, with some additional benefit from the addition of IV MTX 5. Historically, the introduction of CRT was a major breakthrough, but there is no evidence that higher dose CRT offers any advantage. Although the addition of CRT to short-term IT (≤8 doses) treatment showed benefit in early trials, alternative therapy, such as giving additional IT doses or IV MTX can be as effective in terms of EFS and OS. In CCG-105, the omission of CRT diminished EFS for those receiving standard (minimal) therapy but not for those receiving more intensive BFM-based treatment 13. Therefore the ability to omit CRT depends on systemic as well as IT therapy. Even if CRT is used, there was some evidence, mainly in T-lineage disease, that the addition of IV MTX to CRT might improve EFS further for some patients.

There was no direct evidence on the comparative effectiveness of IT MTX, DIT, and TIT in the previous meta-analysis. The CCG-1952 trial has now provided some evidence on TIT versus IT MTX, which has suggested that other comparison groups should be split, and this has been confirmed by heterogeneity tests between the revised subgroups. On the other hand, different IT therapies are grouped together in the comparisons between trials with CRT and those with IV MTX on the assumption that small differences in IT MTX, DIT, TIT therapies will have minor effects compared with those of CRT and IV MTX. Direct evidence comparing doses of IV MTX has also become available.

When neither CRT nor IV MTX are used, CCG-1952 study showed that, compared with IT MTX, TIT reduced the frequency of isolated CNS relapse. The impact of TIT on isolated CNS relapse was especially pronounced in patients with CNS2 status such that it reduced 6-year cumulative risk to 7.7 ± 5.3% as compared to 23.0 ± 9.5% among those treated with IT MTX (P = 0.004); the 6-year cumulative risk of isolated CNS relapse among patients with CNS1 status was 3.1 ± 1.0% versus 5.1 ± 1.2% between those treated with TIT or IT MTX (P = 0.03) 10. However, TIT treatment was unexpectedly associated with an increased frequency of bone marrow and testicular relapse, resulting in similar EFS and poorer OS. One explanation for this seemingly paradoxical finding is that cytarabine or hydrocortisone in TIT interferes with egress of MTX from cerebrospinal fluid into blood such that patients receiving TIT had less systemic exposure to MTX 10. Another explanation is that CNS relapse and other relapses are competing events so that improved CNS control by TIT might have led to leukemic relapse in other sites. In this regard, adding IV MTX to TIT improved outcome by reducing both CNS and non-CNS relapses, whereas adding IV to IT had little effect. Only one trial compared IV MTX plus IT with TIT, with no EFS or OS difference. It may be that more effective systemic therapy is needed before the full benefit of TIT can be realized. COG is re-examining the question of TIT in the B-precursor higher risk trial, AALL1131. All patients will receive IV MTX at 5 g/m2 with leucovorin and the more favorable patients by presenting features and response will be randomized to receive IT MTX versus TIT.

That higher dose of IV MTX failed to significantly improve EFS in some studies might be due to excessive leucovorin rescue 24, 57, 58. In this regard, it is of special interest that even lower dose IV MTX, without leucovorin, on CCG-1991 (100–300 mg/m2) can reduce CNS relapse 59. This trial was in NCI standard risk patients and compared two courses of interim maintenance with five doses of vincristine plus IV MTX at 100 mg/m2 escalated by 50 mg/m2 every 10 days for four doses as tolerated, versus oral mercaptopurine, oral methotrexate plus ten doses of dexamethasone at 6 mg/m2 and reported an EFS advantage with escalating IV MTX. However, the recent COG AALL0232 trial in NCI high risk patients up to the age of 30 years shows a substantial EFS advantage for IV MTX at 5 g/m2 with leucovorin rescue compared with asparaginase plus escalating IV MTX without rescue 60, 61. This trial also randomized between dexamethasone and prednisone. The final report is not yet available, but there appears to be an interaction suggesting EFS benefit only for the high dose IV MTX plus dexamethasone combination. In this regard, when high dose IV MTX is used, attention needs to be paid to the leucovorin rescue, keeping it to the minimum necessary.

Despite impressive gains from the treatment of childhood ALL, there is still a need to improve management of CNS disease so that CRT can be avoided in all children. In this regard, both intrathecal and systemic therapy must be optimized in order to achieve the goal. Dexamethasone, high dose cytarabine and asparaginase may all be relevant. With improved systemic therapy, future studies should determine the optimal number of intrathecal therapy because this treatment modality can also adversely affect neurocognitive function. However, a certain number of intrathecal therapy doses are necessary because high dose methotrexate alone, even at very high dose (33.6 g/m2), was inadequate for CNS control 62. To answer outstanding questions it would be helpful for future trials to be designed so that randomized comparisons address single drugs as far as possible.

No clear subgroup differences exist, but information is limited, particularly for T-lineage ALL. An effective CNS-directed treatment strategy without concomitant adequate systemic therapy may lead to increased bone marrow relapse and poorer OS. Thus, the combination of CNS and systemic effects, as well as outcome post-relapse, need to always be borne in mind.

Acknowledgements

We thank the following for providing trial data: French ALL Cooperative group: A. Baruchel, M. Auclerc; Instituto Nacional de Enfermedades Neoplasicas (INEN), Peru: C. Perez, A. Solidaro; Jena University, Germany: F. Zintl, I. Schiller; Tokyo Children's Cancer Study Group, Japan: S. Nakazawa, M. Tsuchida.

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