Chromatographic analysis of simple phenols in some species from the genus Salix
Version of Record online: 23 MAR 2010
Copyright © 2010 John Wiley & Sons, Ltd.
Volume 21, Issue 5, pages 463–469, September/October 2010
How to Cite
Pobłocka-Olech, L., Krauze-Baranowska, M., Głód, D., Kawiak, A. and Łojkowska, E. (2010), Chromatographic analysis of simple phenols in some species from the genus Salix. Phytochem. Anal., 21: 463–469. doi: 10.1002/pca.1220
- Issue online: 25 AUG 2010
- Version of Record online: 23 MAR 2010
- Manuscript Accepted: 1 FEB 2010
- Manuscript Revised: 29 JAN 2010
- Manuscript Received: 24 APR 2009
- Phenolic acids;
- willow bark;
- comet assay
Introduction – Salicis Cortex, made from willow bark is a herbal remedy, which is standardised based on the content of salicin, a compound with analgesic and antiphlogistic properties. However, clinical trials suggest that other compounds also present in Salicis Cortex can contribute to the pharmacological effects.
Objective – To characterise the composition of phenolic acids in the barks of different species and clones from the genus Salix by use of chromatographic methods—HPTLC and HPLC.
Methodology – The phenolic acid composition was analysed by MGD (multiple gradient development)–HPTLC technique. The separation was performed on HPTLC Diol plates with gradient elution using a mixture of chloroform:hexane:ethyl acetate with increasing concentration of ethyl acetate from 10 to 25%. Derivatisation with thymol reagent was employed for the first time for specific detection of phenolic acids containing methoxyl groups.
Results – The presence of all phenolic acids previously reported in the genus Salix was confirmed, namely p-hydroxybenzoic, vanillic, cinnamic, p-coumaric, ferulic and caffeic acids. Furthermore, pyrocatechol as a constituent of willow bark was revealed. The highest concentration of this compound was observed in the S. purpurea bark (2.25 mg/g).
Conclusion – The presence of a relatively high content of pyrocatechol in Salix species may raise doubts about the safe application of this herbal medicine. Copyright © 2010 John Wiley & Sons, Ltd.