This article is a U.S. Government work and is in the public domain in the U.S.A.
A comparison of alternative sample preparation procedures for the analysis of swainsonine using LC-MS/MS†
Article first published online: 26 AUG 2010
Copyright © 2010 John Wiley & Sons, Ltd.
Volume 22, Issue 2, pages 124–127, March/April 2011
How to Cite
Gardner, D. R. and Cook, D. (2011), A comparison of alternative sample preparation procedures for the analysis of swainsonine using LC-MS/MS. Phytochem. Anal., 22: 124–127. doi: 10.1002/pca.1257
- Issue published online: 18 FEB 2011
- Article first published online: 26 AUG 2010
- Manuscript Accepted: 15 JUN 2010
- Manuscript Revised: 11 JUN 2010
- Manuscript Received: 11 MAY 2010
- Solid-phase extraction;
- cation exchange;
Introduction – Swainsonine, a polyhydroxy indolizidine alkaloid and known glycosidase inhibitor, is found in a number of different plants that cause a lysosomal storage disease known as locoism in the western USA. Most recently swainsonine has been analysed by LC-MS/MS after sample extraction and preparation from ion-exchange resins.
Objective – To compare previously published sample preparation procedures with several new alternative procedures to provide methods using either commercially available solid-phase extraction equipment or procedures which significantly reduce sample preparation time.
Methodology – A previously reported and validated sample preparation method using ion-exchange resin was compared with methods using a commercially available solid-phase extraction cartridge, a solvent partitioning procedure or a single solvent extraction procedure using one of two solvents. Twenty different plant samples of varying swainsonine concentrations were prepared in triplicate and analysed by LC-MS/MS. The measured concentration of swainsonine was then statistically compared between methods.
Results – There were no statistically significant differences found between four of the five different sample preparation methods tested.
Conclusion – A commercially available SPE cartridge can be used to replace the previously used ion-exchange resin for swainsonine analysis. For very rapid analyses the SPE procedure can be eliminated and a simple, single solvent extraction step used for sample preparation. Published in 2010 by John Wiley & Sons, Ltd.