Bio-assay Guided Isolation of α-Glucosidase Inhibitory Constituents from Hibiscus Mutabilis Leaves
Version of Record online: 12 DEC 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Volume 23, Issue 5, pages 421–425, September/October 2012
How to Cite
Kumar, D., Kumar, H., Vedasiromoni, J. R. and Pal, B. C. (2012), Bio-assay Guided Isolation of α-Glucosidase Inhibitory Constituents from Hibiscus Mutabilis Leaves. Phytochem. Anal., 23: 421–425. doi: 10.1002/pca.1375
- Issue online: 10 AUG 2012
- Version of Record online: 12 DEC 2011
- Manuscript Accepted: 14 OCT 2011
- Manuscript Revised: 30 SEP 2011
- Manuscript Received: 13 MAY 2011
- HPLC method validation;
- caffeic acid;
- ferulic acid;
- Hibiscus mutabilis
The increasing demand for natural-product-based medicines and health-care products for the management of diabetes encouraged investigation of this commonly available Indian plant.
To establish the anti-diabetic (α-glucosidase inhibitory) activity of H. mutabilis leaf extract, isolate and identify the constituents responsible for the activity, and validate a HPLC method for quantification of the active constituents for standardisation of the extract.
Material and methods
The methanolic extract of leaves was partitioned between water, n-butanol and ethyl acetate. Bio-assay guided fractionation, based on inhibition of α-glucosidase, allowed isolation and identification of the active components. The active components were quantified using RP-HPLC-DAD validated for linearity, limit of detection, limit of quantification, precision, accuracy and robustness for this plant extract and the partitioned fractions.
Ferulic acid and caffeic acid were identified as the α-glucosidase inhibitors present in H. mutabilis. They were partitioned into an ethyl acetate fraction. The HPLC-DAD calibration curve showed good linearity (r2 > 0.99). For the recovery studies the %RSD was less than 2%. The interday and intraday variations were found to be less than 4% RSD for retention time and response.
The identification of α-glucosidase inhibition activity in H. mutabilis supports further investigations into the possible use of the plant for the management of diabetes. The HPLC method validated for these extracts will be useful in future research with the plant. Copyright © 2011 John Wiley & Sons, Ltd.