Rapid Profiling of Intact Glucosinolates in Arabidopsis Leaves by UHPLC-QTOFMS Using a Charged Surface Hybrid Column
Version of Record online: 9 FEB 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 23, Issue 5, pages 520–528, September/October 2012
How to Cite
Glauser, G., Schweizer, F., Turlings, T. C. J. and Reymond, P. (2012), Rapid Profiling of Intact Glucosinolates in Arabidopsis Leaves by UHPLC-QTOFMS Using a Charged Surface Hybrid Column. Phytochem. Anal., 23: 520–528. doi: 10.1002/pca.2350
- Issue online: 10 AUG 2012
- Version of Record online: 9 FEB 2012
- Manuscript Accepted: 27 DEC 2011
- Manuscript Revised: 23 DEC 2011
- Manuscript Received: 7 OCT 2011
- Arabidopsis thaliana;
The analysis of glucosinolates (GS) is traditionally performed by reverse-phase liquid chromatography coupled to ultraviolet detection after a time-consuming desulphation step, which is required for increased retention. Simpler and more efficient alternative methods that can shorten both sample preparation and analysis are much needed.
To evaluate the feasibility of using ultrahigh-pressure liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC-QTOFMS) for the rapid profiling of intact GS.
A simple and short extraction of GS from Arabidopsis thaliana leaves was developed. Four sub-2 µm reverse-phase columns were tested for the rapid separation of these polar compounds using formic acid as the chromatographic additive. High-resolution QTOFMS was used to detect and identify GS.
A novel charged surface hybrid (CSH) column was found to provide excellent retention and separation of GS within a total running time of 11 min. Twenty-one GS could be identified based on their accurate mass as well as isotopic and fragmentation patterns. The method was applied to determine the changes in GS content that occur after herbivory in Arabidopsis. In addition, we evaluated its applicability to the profiling of other Brassicaceae species.
The method developed can profile the full range of GS, including the most polar ones, in a shorter time than previous methods, and is highly compatible with mass spectrometric detection. Copyright © 2012 John Wiley & Sons, Ltd.