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Research Article
HPLC Quantitative Analysis of Insecticidal Didehydrostemofoline and Stemofoline in Stemona collinsiae Root Extracts
Article first published online: 6 MAR 2012
DOI: 10.1002/pca.2356
Copyright © 2012 John Wiley & Sons, Ltd.
Additional Information
How to Cite
Kongkiatpaiboon, S. and Gritsanapan, W. (2012), HPLC Quantitative Analysis of Insecticidal Didehydrostemofoline and Stemofoline in Stemona collinsiae Root Extracts. Phytochem. Anal., 23: 554–558. doi: 10.1002/pca.2356
Publication History
- Issue published online: 10 AUG 2012
- Article first published online: 6 MAR 2012
- Manuscript Accepted: 23 JAN 2012
- Manuscript Revised: 18 JAN 2012
- Manuscript Received: 21 AUG 2011
Funded by
- Royal Golden Jubilee PhD Program. Grant Number: PHD/0139/2550
- Abstract
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- References
- Cited By
Keywords:
- HPLC analysis;
- didehydrostemofoline;
- stemofoline;
- Stemona collinsiae;
- Stemonaceae
ABSTRACT
Introduction
Stemona spp. have been traditionally used as natural pesticides and medicinal plants. Stemona collinsiae Craib has been of interest for its insecticidal activity, which has been supported by many scientific research studies. The roots contain didehydrostemofoline and stemofoline alkaloids as active components.
Objective
To develop and validate a HPLC method for the quantitative analysis of didehydrostemofoline and stemofoline in S. collinsiae root extracts.
Methodology
HPLC was carried out using a Hypersil BDS C18-column eluted with methanol:1 m m ammonium acetate (55:45) with a flow rate of 1 mL/min and detection at 295 nm. Method validation was performed to assure its linearity, precision, accuracy and limits of detection and quantitation.
Results
Didehydrostemofoline and stemofoline showed a linear relationship within the range of 0.5–432.4 and 0.5–188.4 µg/mL, respectively. The method was shown to be precise with RSD < 2%. The average recovery of didehydrostemofoline and stemofoline were 98.80 and 99.97%, respectively. Eight samples of S. collinsiae root extracts were analysed and the average contents of didehydrostemofoline and stemofoline were 0.78 and 0.048% w/w, respectively.
Conclusion
The HPLC method developed was appropriate for the analysis of didehydrostemofoline and stemofoline in S. collinsiae root extracts. This work would be useful as a guide for the standardisation of S. collinsiae root extract raw materials and their products as natural pesticides. Copyright © 2012 John Wiley & Sons, Ltd.