HPLC Quantitative Analysis of Insecticidal Didehydrostemofoline and Stemofoline in Stemona collinsiae Root Extracts
Article first published online: 6 MAR 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 23, Issue 5, pages 554–558, September/October 2012
How to Cite
Kongkiatpaiboon, S. and Gritsanapan, W. (2012), HPLC Quantitative Analysis of Insecticidal Didehydrostemofoline and Stemofoline in Stemona collinsiae Root Extracts. Phytochem. Anal., 23: 554–558. doi: 10.1002/pca.2356
- Issue published online: 10 AUG 2012
- Article first published online: 6 MAR 2012
- Manuscript Accepted: 23 JAN 2012
- Manuscript Revised: 18 JAN 2012
- Manuscript Received: 21 AUG 2011
- Royal Golden Jubilee PhD Program. Grant Number: PHD/0139/2550
- HPLC analysis;
- Stemona collinsiae;
Stemona spp. have been traditionally used as natural pesticides and medicinal plants. Stemona collinsiae Craib has been of interest for its insecticidal activity, which has been supported by many scientific research studies. The roots contain didehydrostemofoline and stemofoline alkaloids as active components.
To develop and validate a HPLC method for the quantitative analysis of didehydrostemofoline and stemofoline in S. collinsiae root extracts.
HPLC was carried out using a Hypersil BDS C18-column eluted with methanol:1 m m ammonium acetate (55:45) with a flow rate of 1 mL/min and detection at 295 nm. Method validation was performed to assure its linearity, precision, accuracy and limits of detection and quantitation.
Didehydrostemofoline and stemofoline showed a linear relationship within the range of 0.5–432.4 and 0.5–188.4 µg/mL, respectively. The method was shown to be precise with RSD < 2%. The average recovery of didehydrostemofoline and stemofoline were 98.80 and 99.97%, respectively. Eight samples of S. collinsiae root extracts were analysed and the average contents of didehydrostemofoline and stemofoline were 0.78 and 0.048% w/w, respectively.
The HPLC method developed was appropriate for the analysis of didehydrostemofoline and stemofoline in S. collinsiae root extracts. This work would be useful as a guide for the standardisation of S. collinsiae root extract raw materials and their products as natural pesticides. Copyright © 2012 John Wiley & Sons, Ltd.