Simultaneous Extraction and Separation of Flavonols and Flavones from Chamaecyparis obtusa by Multi-phase Extraction using an Ionic Liquid-modified Microsphere Polymer
Article first published online: 6 MAR 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 23, Issue 6, pages 576–581, November/December 2012
How to Cite
Tian, M., Bi, W. and Row, K. H. (2012), Simultaneous Extraction and Separation of Flavonols and Flavones from Chamaecyparis obtusa by Multi-phase Extraction using an Ionic Liquid-modified Microsphere Polymer. Phytochem. Anal., 23: 576–581. doi: 10.1002/pca.2357
- Issue published online: 13 OCT 2012
- Article first published online: 6 MAR 2012
- Manuscript Accepted: 25 JAN 2012
- Manuscript Revised: 19 JAN 2012
- Manuscript Received: 18 DEC 2011
- Multi-phase extraction;
- ionic liquid;
- microsphere polymer;
- Chamaecyparis obtusa
Flavonols and flavones, especially quercitrin, myricetin and amentoflavone, are the main anti-bacterial and anti-cancer compounds in Chamaecyparis obtuse. Multi-phase extraction is a new method that can extract and separate target compounds simultaneously.
An amino ionic liquid immobilised microsphere polymer was used as a multi-phase extraction sorbent to extract and separate quercitrin, myricetin and amentoflavone from Chamaecyparis obtusa.
The sorbent and Chamaecyparis obtusa powder were packed into a single cartridge. Using a fixed volume of methanol with five repetitions, the target compounds were extracted from the powder to the sorbent. The sorbent was then washed with n-hexane to remove any interfering species and the target compounds were eluted sequentially using water, methanol and methanol containing 1% acetic acid (vol.).
Under the optimised conditions, 0.45 mg/g of quercitrin, 0.18 mg/g of myricetin and 0.12 mg/g of amentoflavone from 2.0 g of powder were obtained by multi-phase extraction using 0.3 g of sorbent.
The method described has a low deviation error, requires a small amount of solvent and is highly selective and reproducible. Copyright © 2012 John Wiley & Sons, Ltd.