Concentration Kinetics of Secoisolariciresinol Diglucoside and its Biosynthetic Precursor Coniferin in Developing Flaxseed
Version of Record online: 12 JUN 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 24, Issue 1, pages 41–46, January/February 2013
How to Cite
Fang, J., Ramsay, A., Paetz, C., Tatsis, E. C., Renouard, S., Hano, C., Grand, E., Fliniaux, O., Roscher, A., Mesnard, F. and Schneider, B. (2013), Concentration Kinetics of Secoisolariciresinol Diglucoside and its Biosynthetic Precursor Coniferin in Developing Flaxseed. Phytochem. Anal., 24: 41–46. doi: 10.1002/pca.2377
- Issue online: 6 DEC 2012
- Version of Record online: 12 JUN 2012
- Manuscript Accepted: 30 APR 2012
- Manuscript Revised: 27 APR 2012
- Manuscript Received: 2 FEB 2012
- secoisolariciresinol diglucoside;
- Linum usitatissimum
In the plant kingdom, flaxseed (Linum usitatissimum L.) is the richest source of secoisolariciresinol diglucoside (SDG), which is of great interest because of its potential health benefits for human beings. The information about the kinetics of SDG formation during flaxseed development is rare and incomplete.
In this study, a reversed-phase high-performance liquid chromatography–diode array detection (HPLC-DAD) method was developed to quantify SDG and coniferin, a key biosynthetic precursor of SDG in flaxseed.
Seeds from different developmental stages, which were scaled by days after flowering (DAF), were harvested. After alkaline hydrolysis, the validated HPLC method was applied to determine SDG and coniferin concentrations of flaxseed from different developing stages.
Coniferin was found in the entire capsule as soon as flowering started and became undetectable 20 DAF. SDG was detected 6 DAF, and the concentration increased until maturity. On the other hand, the SDG amount in a single flaxseed approached the maximum around 25 DAF, before desiccation started. Concentration increase between 25 DAF and 35 DAF can be attributed to corresponding seed weight decrease.
The biosynthesis of coniferin is not synchronous with that of SDG. Hence, the concentrations of SDG and coniferin change during flaxseed development. Copyright © 2012 John Wiley & Sons, Ltd.