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Supercritical CO2 Assisted Extraction and LC–MS Identification of Picroside I and Picroside II from Picrorhiza kurroa

Authors

  • Ajit A. Patil,

    1. Pharmaceutical Technology Division, Department of Chemical Technology, Dr Babasaheb Ambedkar Marathwada University, Aurangabad, MH, India
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  • Bhusari S. Sachin,

    1. Pharmaceutical Technology Division, Department of Chemical Technology, Dr Babasaheb Ambedkar Marathwada University, Aurangabad, MH, India
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  • Devanand B. Shinde,

    1. Pharmaceutical Technology Division, Department of Chemical Technology, Dr Babasaheb Ambedkar Marathwada University, Aurangabad, MH, India
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  • Pravin S. Wakte

    Corresponding author
    • Pharmaceutical Technology Division, Department of Chemical Technology, Dr Babasaheb Ambedkar Marathwada University, Aurangabad, MH, India
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Correspondence to: Pravin S. Wakte, Pharmaceutical Technology Division, Department of Chemical Technology, Dr Babasaheb Ambedkar Marathwada University, Aurangabad-431004, India. Email: pharmtech.udct@gmail.com

ABSTRACT

Introduction

Picroside I and picroside II have been studied intensively because of their pharmacological actions and clinical applications. Numerous methods have been reported for extracting picroside I and picroside II from Picrorrhiza. kurroa rhizomes. This is the first report of picroside I and picroside II extraction using the supercritical carbon dioxide assisted extraction technique.

Objective

To develop supercritical carbon dioxide assisted extraction and LC–MS identification of picroside I and picroside II from the Picrorrhiza kurroa Royle rhizomes.

Methodology

Surface response methodology based on 33 fractional factorial design was used to extract picroside I and picroside II from P. kurroa rhizomes. The effects of various process factors, namely temperature (40–80°C), pressure (25–35 MPa) and co-solvent (methanol) concentration (0–10% v/v) on extraction yield of the two compounds were evaluated. The picroside I and picroside II contents were determined using validated LC–MS methodology.

Results

The maximum yield of picroside I (32.502 ± 1.131 mg/g) and picroside II (9.717 ± 0.382 mg/g) was obtained at the 10% v/v co-solvent concentration, 40°C temperature and 30 MPa pressure. The conventional Soxhlet assisted methanol extract of P. kurroa powder resulted in 36.743 ± 1.75 and 11.251 ± 0.54 mg/g yield of picroside I and picroside II, respectively.

Conclusion

Variation of concentration and extraction time showed a significant effect on the picroside I and picroside II yield. Supercritical carbon dioxide assisted extraction using methanol as a co-solvent is an efficient and environmentally sustainable method for extracting picroside I and picroside II from P. kurroa rhizomes. Copyright © 2012 John Wiley & Sons, Ltd.

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