Multi-phase Extraction of Glycoraphanin from Broccoli Using Aminium Ionic Liquid-based Silica
Article first published online: 9 JUL 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 24, Issue 1, pages 81–86, January/February 2013
How to Cite
Tian, M., Bi, W. and Row, K. H. (2013), Multi-phase Extraction of Glycoraphanin from Broccoli Using Aminium Ionic Liquid-based Silica. Phytochem. Anal., 24: 81–86. doi: 10.1002/pca.2386
- Issue published online: 6 DEC 2012
- Article first published online: 9 JUL 2012
- Manuscript Revised: 8 JUN 2012
- Manuscript Accepted: 8 JUN 2012
- Manuscript Received: 25 APR 2012
- Ministry of Education, Science and Technology. Grant Number: 2012–0005250
- Aminium ionic liquid-based silica;
- multi-phase extraction;
Glucosinolates, a class of phytochemicals found in broccoli, have attracted recent interest due to the potential health benefits associated with their dietary intake. Glucoraphanin, the most common glucosinolate in broccoli can be converted to a known cancer chemopreventive agent. Multi-phase extraction in solid-phase extraction cartridges was developed to simultaneously extract and separate this compound.
Multi-phase extraction with functionalised ionic liquid-based silica as a sorbent was used to simultaneously extract and separate glucoraphanin from broccoli.
The sorbent and broccoli sample were packed into a single cartridge, and a fixed volume of water was then used to extract and remove the target compound from the sample to the sorbent over 15 repetitions. The sorbent was then washed with n-hexane to remove any interference and the target compound was eluted with water–1% acetic acid (vol.).
Under the optimised condition, 0.038 mg/g of glucoraphanin was obtained by multi-phase extraction with 0.2 g of sorbent.
The adsorption isotherm allowed investigation of the interactions between the sorbent and target compound and provided evidence for the accuracy of this method. The low deviation error, small amount of solvents required, highly selective separation and stability of the method justify further research. Copyright © 2012 John Wiley & Sons, Ltd.