Simple and Reliable Methods for the Determination of Three Steroidal Glycosides in the Eight Species of Solanum by Reversed-phase HPLC Coupled with Diode Array Detection
Version of Record online: 11 JUL 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 24, Issue 1, pages 87–92, January/February 2013
How to Cite
Maurya, A., Manika, N., Verma, R. K., Singh, S. C. and Srivastava, S. K. (2013), Simple and Reliable Methods for the Determination of Three Steroidal Glycosides in the Eight Species of Solanum by Reversed-phase HPLC Coupled with Diode Array Detection. Phytochem. Anal., 24: 87–92. doi: 10.1002/pca.2387
- Issue online: 6 DEC 2012
- Version of Record online: 11 JUL 2012
- Manuscript Accepted: 10 JUN 2012
- Manuscript Revised: 7 JUN 2012
- Manuscript Received: 15 MAR 2012
- Reverse-phase HPLC;
- indioside D;
Solanum species are important ingredients of many traditional Indian medicines and thus the quality control of their herbal formulations is of paramount concern.
To establish a simple and effective high-performance liquid chromatographic (HPLC) method to evaluate the quality of Solanum species and their herbal formulations.
A rapid, simple, sensitive, robust and reproducible HPLC method was developed for the determination of three steroidal glycosides (SG); indioside D, solamargine and α-solanine in eight species of the genus Solanum. The analytes were separated on a monolithic performance RP-18e column (100 mm × 4.6 mm i.d.) using a gradient elution of acetonitile–water containing 0.1% trifluoroacetic acid (TFA) as the mobile phase with a flow rate 0.4 mL/min and UV detection at λ 210 nm.
The method was linear over the range 3–15 µg/mL (r > 9994). Accuracy, precision and repeatability were all within the required limits. The mean recoveries measured at the three concentrations were higher than 98.8% with RSD < 2% for the targets.
The established method is simple and can be used as a tool for quality control of plant material or herbal formulation containing SG. Copyright © 2012 John Wiley & Sons, Ltd.