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Keywords:

  • Reverse-phase HPLC;
  • indioside D;
  • solamargine;
  • α-solanine;
  • Solanum

ABSTRACT

Introduction

Solanum species are important ingredients of many traditional Indian medicines and thus the quality control of their herbal formulations is of paramount concern.

Objective

To establish a simple and effective high-performance liquid chromatographic (HPLC) method to evaluate the quality of Solanum species and their herbal formulations.

Methodology

A rapid, simple, sensitive, robust and reproducible HPLC method was developed for the determination of three steroidal glycosides (SG); indioside D, solamargine and α-solanine in eight species of the genus Solanum. The analytes were separated on a monolithic performance RP-18e column (100 mm × 4.6 mm i.d.) using a gradient elution of acetonitile–water containing 0.1% trifluoroacetic acid (TFA) as the mobile phase with a flow rate 0.4 mL/min and UV detection at λ 210 nm.

Results

The method was linear over the range 3–15 µg/mL (r> 9994). Accuracy, precision and repeatability were all within the required limits. The mean recoveries measured at the three concentrations were higher than 98.8% with RSD < 2% for the targets.

Conclusion

The established method is simple and can be used as a tool for quality control of plant material or herbal formulation containing SG. Copyright © 2012 John Wiley & Sons, Ltd.