Influence of Bracken Fern (Pteridium caudatum L. Maxon) Pre-treatment on Extraction Yield of Illudane Glycosides and Pterosins
Version of Record online: 20 NOV 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Volume 24, Issue 4, pages 290–295, July/August 2013
How to Cite
Cáceres-Peña, Y. C., Naya, M., Calcagno-Pissarelli, M. P. and Alonso-Amelot, M. E. (2013), Influence of Bracken Fern (Pteridium caudatum L. Maxon) Pre-treatment on Extraction Yield of Illudane Glycosides and Pterosins. Phytochem. Anal., 24: 290–295. doi: 10.1002/pca.2409
- Issue online: 18 JUN 2013
- Version of Record online: 20 NOV 2012
- Manuscript Accepted: 2 OCT 2012
- Manuscript Revised: 1 OCT 2012
- Manuscript Received: 10 MAR 2012
- extraction yield;
- plant pre-treatment;
- ptaquiloside Z
Bracken (Pteridium spp) illudane glycosidess are labile biologically active terpenoids that undergo decomposition in mild alkali or acid, heat and enzymatic reactions. Hypothetically, quantitation of these weakly chromophoric carcinogens may be challenged by plant sample preparation procedures that may alter the yield of isolates.
To study the influence of common plant sample pre-treatments on the recovery of Pteridium caudatum illudane glycoside carcinogens, ptaquiloside (1a), caudatoside (1c) and ptaquiloside Z (1d), and associated pterosins A, B and Z (2a, b, c) using HPLC–DAD.
Bracken fronds were divided in equal left/right sections. One section was subjected to high vacuum desiccation (VD) and the other to freeze-drying (FD), air drying at room temperature (AD) for 7 days, air drying at 70 °C for 72 h (HD), or no treatment (fresh frond, FF). Quantitation was achieved by brief hot-water extraction, base-acid transformation of 1a, 1c and 1d to 2a, b, c and HPLC–DAD analysis against standards.
Substantial differences in extraction yields were found for all illudane glycosides in the order FF > FD ≈ VD > AD > HD. Illudane instability to HD was 1c > 1d > 1a. Significant losses also were recorded in yields of Pterosins A, B and Z.
Glycoside extraction suffers from substantial yield loss of all illudane glycosides and indigenous pterosins in all sample pre-treatments studied relative to fresh frond material. Copyright © 2012 John Wiley & Sons, Ltd.