Analysis of Mogroside V in Siraitia grosvenorii with Micelle-mediated Cloud-Point Extraction
Version of Record online: 24 JAN 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Volume 24, Issue 4, pages 381–385, July/August 2013
How to Cite
Deng, F., Liang, X., Yang, L., Liu, Q. and Liu, H. (2013), Analysis of Mogroside V in Siraitia grosvenorii with Micelle-mediated Cloud-Point Extraction. Phytochem. Anal., 24: 381–385. doi: 10.1002/pca.2420
- Issue online: 18 JUN 2013
- Version of Record online: 24 JAN 2013
- Manuscript Accepted: 15 DEC 2012
- Manuscript Revised: 21 NOV 2012
- Manuscript Received: 2 JUL 2012
- National Natural Science Foundation of China. Grant Number: No. 21106152 and No. 21136009
- cloud-point extraction;
- Genapol® X-080;
- mogroside V;
- Siraitia grosvenorii
Mogroside V is the main effective ingredient of Siraitia grosvenorii used as a natural sweet food as well as a traditional Chinese medicine. The sample pre-treatment prior to chromatographic analysis requires large amounts of toxic organic solvents and is time consuming.
To develop an effective and simple method for extracting and determining mogroside V of Siraitia grosvenorii.
Mogroside V was extracted and preconcentrated by micelle-mediated cloud-point extraction with nonionic surfactant isotridecyl poly (ethylene glycol) ether (Genapol® X-080). The obtained solutions containing mogroside V were analysed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The chromatographic separation was performed on a C18-column using gradient elution with acetonitrile and water at 203 nm.
The cloud-point extraction yield was 80.7% while the pre-concentration factor was about 10.8. The limit of detection was 0.75 µg/mL and the limit of quantification was 2 µg/mL. The relative standard deviations for intra- and interday precisions of mogroside V were less than 8.68% and 5.78%, respectively, and the recoveries were between 85.1% and 103.6%.
The HPLC–UV method based on micelle-mediated cloud-point extraction for determination mogroside V in Siraitia grosvenorii was environmentally friendly, simple and sensitive. Copyright © 2013 John Wiley & Sons, Ltd.