Interval Multivariate Curve Resolution in the Dereplication of HPLC–DAD Data from Jatropha gossypifolia
Version of Record online: 10 MAR 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Volume 24, Issue 4, pages 401–406, July/August 2013
How to Cite
Pilon, A. C., Carneiro, R. L., Carnevale Neto, F., da S. Bolzani, V. and Castro-Gamboa, I. (2013), Interval Multivariate Curve Resolution in the Dereplication of HPLC–DAD Data from Jatropha gossypifolia. Phytochem. Anal., 24: 401–406. doi: 10.1002/pca.2423
- Issue online: 18 JUN 2013
- Version of Record online: 10 MAR 2013
- Manuscript Accepted: 17 JAN 2013
- Manuscript Revised: 15 JAN 2013
- Manuscript Received: 9 OCT 2012
- Jatropha gossypifolia
Jatropha gossypifolia has been used quite extensively by traditional medicine for the treatment of several diseases in South America and Africa. This medicinal plant has therapeutic potential as a phytomedicine and therefore the establishment of innovative analytical methods to characterise their active components is crucial to the future development of a quality product.
To enhance the chromatographic resolution of HPLC–UV–diode-array detector (DAD) experiments applying chemometric tools.
Crude leave extracts from J. gossypifolia were analysed by HPLC–DAD. A chromatographic band deconvolution method was designed and applied using interval multivariate curve resolution by alternating least squares (MCR–ALS).
The MCR–ALS method allowed the deconvolution from up to 117% more bands, compared with the original HPLC–DAD experiments, even in regions where the UV spectra showed high similarity. The method assisted in the dereplication of three C-glycosylflavones isomers: vitexin/isovitexin, orientin/homorientin and schaftoside/isoschaftoside.
The MCR–ALS method is shown to be a powerful tool to solve problems of chromatographic band overlapping from complex mixtures such as natural crude samples. Copyright © 2013 John Wiley & Sons, Ltd.