Identification and Characterisation of Phenolics in Polygonum capitatum by Ultrahigh-Performance Liquid Chromatography with Photodiode Array Detection and Tandem Mass Spectrometry

Authors

  • Shang-Gao Liao,

    1. Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guiyang Medical University, Guiyang, Guizhou, PR China
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    • These authors contributed equally to the work.
  • Li-Juan Zhang,

    1. Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guiyang Medical University, Guiyang, Guizhou, PR China
    2. Guizhou Academy of Instrumental Analysis, Guiyang, Guizhou, PR China
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    • These authors contributed equally to the work.
  • Fa Sun,

    1. Department of Urinary Surgery, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou, PR China
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  • Zhen Wang,

    1. Department of Urinary Surgery, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou, PR China
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  • Xun He,

    1. Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guiyang Medical University, Guiyang, Guizhou, PR China
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  • Ai-Min Wang,

    1. Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guiyang Medical University, Guiyang, Guizhou, PR China
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  • Yong-Jun Li,

    1. Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guiyang Medical University, Guiyang, Guizhou, PR China
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  • Yong Huang,

    1. Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guiyang Medical University, Guiyang, Guizhou, PR China
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  • Yan-Yu Lan,

    1. Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guiyang Medical University, Guiyang, Guizhou, PR China
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  • Bo-Li Zhang,

    Corresponding author
    1. Tianjin University of Traditional Chinese Medicine, Tianjin, PR China
    • Correspondence to: B.-L. Zhang, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, PR China and Y.-L. Wang, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guiyang Medical University, 9 Beijing Road, Guiyang, Guizhou, 550004, PR China. Email: zhangboli@tjutc.com; ylwang_gmc@163.com

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  • Yong-Lin Wang

    Corresponding author
    1. Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guiyang Medical University, Guiyang, Guizhou, PR China
    • Correspondence to: B.-L. Zhang, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, PR China and Y.-L. Wang, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, Guiyang Medical University, 9 Beijing Road, Guiyang, Guizhou, 550004, PR China. Email: zhangboli@tjutc.com; ylwang_gmc@163.com

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ABSTRACT

Introduction

Polygonum capitatum is a well-known Chinese medicinal plant widely used by the Miao people for the treatment of various urologic disorders. Previous investigations have shown the presence of various types of phenolics. Our ultrahigh-performance liquid chromatography with photodiode array detection and mass spectrometry (UPLC–PDA–MS) analysis indicated that flavonoid glycosides and polyphenolic glycosides were its major constituents and quite a number of phenolic compounds have not yet been identified. Identification or characterisation of the major compounds of this plant will contribute to the scientific understanding of the medicinal plant and the authentication of the plant material and its pharmaceutical preparations.

Objective

To develop an efficient method for the identification and structural characterisation of the major compounds present in P. capitatum.

Methods

Elution of the 70% ethanol extract of P. capitatum by 80% ethanol on a D101 macroporous resin column afforded a phenolics-enriched fraction, separation of which by Sephadex LH-20 column chromatography eluted with absolute ethanol gave a tannin-free phenolic fraction (TFPF). Compounds present in TFPF were identified and structurally characterised by UPLC–PDA–ESI–MS/MS.

Results

An amino acid and 40 phenolics including a number of flavonoid glycosides and polyphenolic glycosides were identified or structurally characterised in TFPF. Among these compounds, four were new and 19 were described in the plant for the first time.

Conclusion

The study showed that TFPF of P. capitatum contained flavonoid glycosides and polyphenolic glycosides as its major principles. Polyphenolic glycosides were perhaps the most typical chemical markers of P. capitatum. Copyright © 2013 John Wiley & Sons, Ltd.

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