A Proposed Biosynthetic Pathway of Picrosides Linked through the Detection of Biochemical Intermediates in the Endangered Medicinal Herb Picrorhiza kurroa
Article first published online: 21 MAY 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Volume 24, Issue 6, pages 598–602, November/December 2013
How to Cite
Kumar, V., Sood, H., Sharma, M. and Chauhan, R. S. (2013), A Proposed Biosynthetic Pathway of Picrosides Linked through the Detection of Biochemical Intermediates in the Endangered Medicinal Herb Picrorhiza kurroa. Phytochem. Anal., 24: 598–602. doi: 10.1002/pca.2437
- Issue published online: 24 OCT 2013
- Article first published online: 21 MAY 2013
- Manuscript Accepted: 28 FEB 2013
- Manuscript Revised: 25 FEB 2013
- Manuscript Received: 26 DEC 2012
- biosynthetic pathway;
- Picrorhiza kurroa
Picrorhiza kurroa Royle ex Benth is an important medicinal herb used in the preparation of several herbal drug formulations due to the presence of picroside-I (P-I) and picroside-II (P-II) along with other iridoid-glucosides derivatives.
The endangered status of P. kurroa coupled with lack of information on biosynthesis of P-I and P-II necessitate deciphering the biosynthetic pathway for picrosides.
LC with electrospray ionisation (ESI) and quadrupole time of flight combined with MS/MS was used to detect intermediates and assemble the picrosides biosynthetic pathway in P. kurroa.
The presence of catalpol and aucubin, the major backbone structures of picrosides, along with intermediate metabolites boschnaloside, bartsioside and mussaenosidic acid, was confirmed in ESI negative mode with pseudomolecular ion peaks, that is, m/z 361, m/z 343, m/z 345, m/z 329 and m/z 375 ions and their fragmentation patterns.
The picrosides biosynthetic pathway is expected to provide a reliable platform towards understanding the molecular components (genes/enzymes) of P-I and P-II biosynthesis in P. kurroa for their eventual utilisation in various applications. Copyright © 2013 John Wiley & Sons, Ltd.