Identification of Nuclear Factor-κB Inhibitors and β2 Adrenergic Receptor Agonists in Chinese Medicinal Preparation Fuzilizhong Pills Using UPLC with Quadrupole Time-of-flight MS
Article first published online: 23 SEP 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Volume 25, Issue 2, pages 113–121, March/April 2014
How to Cite
Dong, L., Cheng, B., Luo, Y., Zhang, N., Duan, H., Jiang, M., Wang, Y., Bai, G. and Luo, G. (2014), Identification of Nuclear Factor-κB Inhibitors and β2 Adrenergic Receptor Agonists in Chinese Medicinal Preparation Fuzilizhong Pills Using UPLC with Quadrupole Time-of-flight MS. Phytochem. Anal., 25: 113–121. doi: 10.1002/pca.2474
- Issue published online: 22 JAN 2014
- Article first published online: 23 SEP 2013
- Manuscript Accepted: 25 JUL 2013
- Manuscript Revised: 23 JUL 2013
- Manuscript Received: 25 FEB 2013
- National Science Foundation of China. Grant Numbers: 81102835, 81173638, 81001682
- Research Funds of Tianjin Medical Universities. Grant Number: 2012KYM12
- 973 program. Grant Number: 2011CB933104
- Dual-bioactivity-integrated fingerprinting;
- β2 adrenergic receptor agonists;
- nuclear factor-κB inhibitors;
- Fuzilizhong pills
Fuzilizhong Pills (FZLZ), a modified form of a famous traditional Chinese medicine (TCM) Lizhong Wan in Treatise on Febrile Diseases and consisting of Panax ginseng C.A.Mey. (Ren Shen), Aconitum carmichaelii Debx. (Fu Zi, Zhi), Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. (Gan Cao), Atractylodes macrocephala Koidz. (Bai Zhu) and Zingiber offcinale Rosc. (Gan Jiang), show strong clinical therapeutic effects for dyspnea and pulmonary oedema. However, the bioactive compounds are still unclear. In this study, FZLZ was analysed using a rapid detection method to identify its anti-inflammatory and spasmolytic constituents.
To develop a simple screening method to detect the anti-inflammatory and spasmolytic constituents of FZLZ.
Ultra-performance liquid chromatography with quadrupole time-of-flight mass spectrometry combined with dual-bioactive (NF-κB and β2-adrenergic receptor) luciferase reporter assay systems was employed.
Two β2-adrenergic receptor agonists (salsolinol and higeramine) and three terpenoidal analogues of NF-κB inhibitors such as ginsenosides derivatives, triperpenoids derivatives and diester-diterpenoid aconitum alkaloid derivatives were characterised. Mesaconitine, flaconitine, ginsenosides Rb2, Rf, Rg2, F1 and Ro were considered to be new NF-κB inhibitors. Furthermore, IL-8 detection by enzyme linked immunosorbent assay confirmed the anti-inflammatory effects of the potential NF-κB inhibitors.
Compared with conventional fingerprints, activity-integrated fingerprints that contain both chemical and bioactive details offer a more comprehensive understanding of the chemical composition of plant materials. This strategy clearly demonstrated that dual bioactivity-integrated fingerprinting is a powerful tool for the improved screening and identification of potential dual-target lead compounds in complex herbal medicines. Copyright © 2013 John Wiley & Sons, Ltd.