One-step Separation and Purification of Two Chromones and One Pyrone from Aloe barbadensis Miller: a Comparison Between Reversed-phase Flash Chromatography and High-speed Counter Current Chromatography
Version of Record online: 5 FEB 2014
Copyright © 2014 John Wiley & Sons, Ltd.
Volume 25, Issue 3, pages 282–288, May/June 2014
How to Cite
Zhong, J.-S., Wan, J.-Z., Ding, W.-J., Wu, X.-F. and Xie, Z.-Y. (2014), One-step Separation and Purification of Two Chromones and One Pyrone from Aloe barbadensis Miller: a Comparison Between Reversed-phase Flash Chromatography and High-speed Counter Current Chromatography. Phytochem. Anal., 25: 282–288. doi: 10.1002/pca.2504
- Issue online: 14 APR 2014
- Version of Record online: 5 FEB 2014
- Manuscript Accepted: 11 DEC 2013
- Manuscript Revised: 9 DEC 2013
- Manuscript Received: 9 OCT 2013
- high-speed counter current chromatography;
- reversed-phase flash chromatography;
- Aloe barbadensis Miller
Chromones and pyrones are the major secondary metabolites of Aloe barbadensis Miller. As they are minor components of the plant, an efficient purification procedure for them is of great importance for promoting their pharmacological studies.
To develop efficient methods for one-step separation and purification of two chromones (5-((S)-2′-oxo-4′-hydroxypentyl)-2-hydroxymethylchromone (1) and 5-((4E)-2′-oxo-pentenyl)-2-hydroxymethylchromone (3)) and one pyrone (aloenin aglycone (2)) from A. barbadensis via reversed-phase flash chromatography (RP-FC) and high-speed counter current chromatography (HSCCC).
The RP-FC separation was performed using methanol:water (26:74, v/v) as the mobile phase at a flow rate of 20 mL/min. A solvent system composed of dichloromethane:methanol:water (3:1.5:1, v/v/v) was used for the HSCCC separation, at a flow rate of 2.0 mL/min.
A one-step RP-FC operation within 110 min was successfully used for the purification of compounds 1 (27.9 mg, 96.5%), 2 (32.4 mg, 98.2%) and 3 (4.1 mg, 99.0%) from 129 mg of crude sample, and a one-step HSCCC separation within 95 min was successfully implemented for the purification of compounds 1 (31.1 mg, 97.6%), 2 (35.8 mg, 96.7%) and 3 (2.7 mg, 98.1%) from 134 mg of crude sample.
The developed procedures were efficient, with low cost and high yield, which would afford sufficient amounts of high-purity compounds for chromatographic purposes and pharmacological activity screening. Copyright © 2014 John Wiley & Sons, Ltd.