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Tentative Characterisation of Iridoids, Phenylethanoid Glycosides and Flavonoid Derivatives from Globularia alypum L. (Globulariaceae) Leaves by LC-ESI-QTOF-MS

Authors

  • Nadia Amessis-Ouchemoukh,

    1. Laboratory of 3BS, Faculty of Life and Nature Sciences, Université Abderahmane Mira, Bejaia, Algeria
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  • Ibrahim M. Abu-Reidah,

    1. Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain
    2. Functional Food Research and Development Centre, Health Science Technological Park, Granada, Spain
    3. Biodiversity and Environmental Research Centre, Til–Nablus, Palestine
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  • Rosa Quirantes-Piné,

    1. Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain
    2. Functional Food Research and Development Centre, Health Science Technological Park, Granada, Spain
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  • Celia Rodríguez-Pérez,

    1. Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain
    2. Functional Food Research and Development Centre, Health Science Technological Park, Granada, Spain
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  • Khodir Madani,

    1. Laboratory of 3BS, Faculty of Life and Nature Sciences, Université Abderahmane Mira, Bejaia, Algeria
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  • Alberto Fernández-Gutiérrez,

    1. Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain
    2. Functional Food Research and Development Centre, Health Science Technological Park, Granada, Spain
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  • Antonio Segura-Carretero

    Corresponding author
    1. Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain
    2. Functional Food Research and Development Centre, Health Science Technological Park, Granada, Spain
    • Correspondence to: A. Segura-Carretero, Department of Analytical Chemistry, Faculty of Science, Granada University, C/Fuentenueva, P. C. 18071, Granada, Spain. E-mail: ansegura@ugr.es

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ABSTRACT

Introduction

Globularia alypum L., belonging to the Globulariaceae family, is a perennial wild shrub found throughout the Mediterranean area, Europe, and Africa. This plant is widely used to treat many diseases, but no previous work on the phytochemical composition of the Algerian G. alypum species has yet been reported.

Objective

To investigate the phytoconstituents of the methanolic extract of G. alypum using an LC-ESI-QTOF-MS method.

Methods

Ground air-dried leaves of G. alypum were macerated with methanol at room temperature for 24 h. The supernatant was filtered and concentrated to dryness under reduced pressure in a rotary evaporator, and extracts were recovered with methanol and filtered. Afterwards, the G. alypum extract was injected into the LC-ESI-QTOF-MS system.

Results

The combined LC–MS/MS led to the tentative characterisation of 63 phytochemicals. In this work, a large number of compounds have been characterised in the leaf-extract analysis of this plant. Among others, 24 iridoids and secoiridoids were found, of which nine compounds have not previously been recorded in G. alypum. Also, nine unusual phenylethanoid glycosides were characterised for the first time in this species.

Conclusion

The method used has proved to be a valued tool for the characterisation of a wide range of compounds from G. alypum leaves. This work constitutes a detailed investigation of the chemical composition of G. alypum leaves, which are widely used in different traditional systems of medicine. Copyright © 2014 John Wiley & Sons, Ltd.

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