Dedicated to Dr. F. J. Novák on the occasion of his 50th birthday.
Flow cytometric analysis of nuclear DNA content in higher plants†
Version of Record online: 2 MAR 2007
Copyright © 1991 John Wiley & Sons, Ltd
Volume 2, Issue 4, pages 143–154, September 1991
How to Cite
Doležel, J. (1991), Flow cytometric analysis of nuclear DNA content in higher plants. Phytochem. Anal., 2: 143–154. doi: 10.1002/pca.2800020402
- Issue online: 2 MAR 2007
- Version of Record online: 2 MAR 2007
- Manuscript Received: 3 NOV 1990
- Manuscript Accepted: 12 JAN 1990
- Flow cytometry;
- higher plants;
- DNA content;
- cell cycle
Methods of flow cytometric analysis of nuclear DNA content were originally developed for human cells. They are based on the use of DNA-specific fluorochromes and on the analysis of the relative fluorescence intensity of stained nuclei. During the last decade this methodology has been adapted to the analysis of plant cells. This article reviews the current status of this rapidly developing field. Special attention is given to preparative techniques, methods of instrument calibration and standardization, and interpretation of data. Multiparameter methods which supplement DNA content analysis are also reviewed.