A single extraction step in the quantitative analysis of arbutin in bearberry (Arctostaphylos uva-ursi) leaves by high-performance liquid chromatography

Authors

  • Irene Parejo,

    1. Department of Natural Products, Plant Biology and Edaphology, Faculty of Pharmacy, University of Barcelona, Av. Diagonal 643, 08028 Barcelona, Catalonia, Spain
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  • Francesc Viladomat,

    1. Department of Natural Products, Plant Biology and Edaphology, Faculty of Pharmacy, University of Barcelona, Av. Diagonal 643, 08028 Barcelona, Catalonia, Spain
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  • Jaume Bastida,

    1. Department of Natural Products, Plant Biology and Edaphology, Faculty of Pharmacy, University of Barcelona, Av. Diagonal 643, 08028 Barcelona, Catalonia, Spain
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  • Carles Codina

    Corresponding author
    1. Department of Natural Products, Plant Biology and Edaphology, Faculty of Pharmacy, University of Barcelona, Av. Diagonal 643, 08028 Barcelona, Catalonia, Spain
    • Department of Natural Products, Plant Biology and Edaphology, Faculty of Pharmacy, University of Barcelona, Av. Diagonal, 643, 08028 Barcelona, Catalonia, Spain
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Abstract

A fast and simple extraction procedure coupled with a simple HPLC method has been developed in order to determine the arbutin content of leaves of Arctostaphylos uva-ursi plants grown at four different geographical sites and collected during two different seasons. Using the optimised analytical system, the arbutin content of bearberry leaves was found to vary from 6.30 to 9.16% expressed on a dry weight basis. Autumn is shown to be a better period than spring for the collection of plant material in order to obtain the highest yield of arbutin. Copyright © 2001 John Wiley & Sons, Ltd.

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