Research Article

Method for the extraction of the volatile compound salicylic acid from tobacco leaf material

  1. Marianne C. Verberne1,*,
  2. Nynke Brouwer1,
  3. Federica Delbianco1,
  4. Huub J. M. Linthorst2,
  5. John F. Bol2,
  6. Robert Verpoorte1

Article first published online: 20 DEC 2001

DOI: 10.1002/pca.615

Issue

Phytochemical Analysis

Phytochemical Analysis

Volume 13, Issue 1, pages 45–50, January/February 2002

Additional Information

How to Cite

Verberne, M. C., Brouwer, N., Delbianco, F., Linthorst, H. J. M., Bol, J. F. and Verpoorte, R. (2002), Method for the extraction of the volatile compound salicylic acid from tobacco leaf material. Phytochem. Anal., 13: 45–50. doi: 10.1002/pca.615

Author Information

  1. 1

    Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research (LACDR), Leiden University, Leiden, The Netherlands

  2. 2

    Division of Plant Virology, Institute for Molecular Plant Sciences (IMP), Leiden University, Leiden, The Netherlands

*Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research (LACDR), Leiden University, Leiden, The Netherlands

Publication History

  1. Issue published online: 14 JAN 2002
  2. Article first published online: 20 DEC 2001
  3. Manuscript Accepted: 1 APR 2001
  4. Manuscript Received: 28 DEC 2000

Funded by

  • Ministry of Economic Affairs
  • Ministry of Education, Culture and Science
  • Ministry of Agriculture, Nature Management and Fishery
  • Van Leersum fund

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (102K)

Keywords:

  • Salicylic acid;
  • 3,4-dihydroxybenzoic acid;
  • extraction;
  • sublimation;
  • acid hydrolysis;
  • recovery

Abstract

Salicylic acid (SA) is a signalling compound in plants which is able to induce systemic acquired resistance. In the analysis of SA in plant tissues, the extraction recovery is often very low and variable. This is mainly caused by sublimation of SA, especially during evaporation of organic solvents. Techniques have been designed in order to overcome this problem. In the first part of the extraction procedure, sublimation of SA was prevented by addition of 0.2 M sodium hydroxide. At a later stage of the extraction procedure, sublimation of SA during solvent evaporation was controlled by the addition of a small amount of HPLC eluent. In this way, recoveries in the range of 71–91% for free SA and 65–79% for acid-hydrolysed SA were obtained. Recoveries could be further optimised by the use of an internal standard to correct for volume changes after the addition of the HPLC eluent. Copyright © 2001 John Wiley & Sons, Ltd.

Get PDF (102K)