High-performance liquid chromatography methods for the analysis of adrenergic amines and flavanones in Citrus aurantium L. var. amara
Version of Record online: 28 JUN 2004
Copyright © 2004 John Wiley & Sons, Ltd.
Volume 15, Issue 4, pages 220–225, July/August 2004
How to Cite
Pellati, F., Benvenuti, S. and Melegari, M. (2004), High-performance liquid chromatography methods for the analysis of adrenergic amines and flavanones in Citrus aurantium L. var. amara. Phytochem. Anal., 15: 220–225. doi: 10.1002/pca.771
- Issue online: 16 JUL 2004
- Version of Record online: 28 JUN 2004
- Manuscript Accepted: 21 JUN 2003
- Manuscript Revised: 12 MAY 2003
- Manuscript Received: 4 FEB 2003
- adrenergic amines;
- dry extracts;
- herbal medicines;
- Citrus aurantium L. var. amara
Reverse-phase HPLC coupled with photodiode array detection was used for the simultaneous separation and determination of naturally occurring adrenergic amines (octopamine, synephrine and tyramine) in fruits and dry extracts of Citrus aurantium L. var. amara and in herbal medicines derived therefrom. Synephrine was the main component in fruits (0.10–0.35%) and in dry extracts (3.00–3.08%) and was present in the range 0.25–0.99% in herbal medicines. Flavanones were analysed in the same samples using a reverse-phase HPLC technique which allowed the identiﬁcation and quantiﬁcation of neoeriocitrin, narirutin, naringin, hesperidin, neohesperidin, naringenin and hesperetin. C. aurantium fruits and derivatives contained mainly glycosylated ﬂavanones: in particular, naringin and neohesperidin were found to be the major ﬂavonoids and their concentrations ranged from 1.80 to 26.30 and from 3.90 to 14.71 mg/g, respectively. The levels of aglycones were very low in all samples tested. Copyright © 2004 John Wiley & Sons, Ltd.