Phytochemical Analysis

Cover image for Vol. 22 Issue 5

September/October 2011

Volume 22, Issue 5

Pages 385–474

  1. Research Articles

    1. Top of page
    2. Research Articles
    1. An Enzyme-Linked Immunosorbant Assay Using Monoclonal Antibody Against Bacoside A3 for Determination of Jujubogenin Glycosides in Bacopa monnieri (L.) Wettst (pages 385–391)

      Charinrat Tothiam, Watoo Phrompittayarat, Waraporn Putalun, Hiroyuki Tanaka, Seiichi Sakamoto, Ikhlas A. Khan and Kornkanok Ingkaninan

      Version of Record online: 16 MAR 2011 | DOI: 10.1002/pca.1293

      Bacopa monnieri (L.) Wettst. (brahmi) is known for memory enhancing activity. Its active compounds are classified as pseudojujubogenin and jujubogenin glycosides. In this study, we developed ELISA using monoclonal antibody (MAb) against bacoside A3, the major jujubogenin glycoside. The validation study showed that the method was precise, accurate and sensitive. Interestingly, the MAb showed no cross reactivity on pseudojujubogenin glycosides. The application of this method for determination of total jujubogenin glycosides in brahmi was demonstrated.

    2. The Optimisation of Analytical Parameters for Routine Profiling of Antioxidants in Complex Mixtures by HPLC Coupled Post-column Derivatisation (pages 392–402)

      Barbara Kusznierewicz, Anita Piasek, Agnieszka Bartoszek and Jacek Namiesnik

      Version of Record online: 4 MAR 2011 | DOI: 10.1002/pca.1294

      The wide application of natural and artificial antioxidants in food, cosmetic and pharmaceutical industry as well as the recognition of the importance of food antioxidants for supporting human health created demand for reliable and industrially applicable methods of determining antioxidative activity. This study presents a ready-to-use methodological toolbox employing the commercial HPLC-coupled post-column derivatisation instrument for routine on-line profiling of antioxidants in complex mixtures and the determination of their TEAC values.

    3. Identification of 2″-Galloylated Flavonol 3-O-Glycosides Accumulating in Developing Leaves of Persimmon (pages 403–410)

      Kayoko Kawakami, Yuki Shibukura, Tomomi Kanno, Tomoko Furuki, Saiko Aketa and Masao Hirayama

      Version of Record online: 16 MAR 2011 | DOI: 10.1002/pca.1295

      Seasonal compositional changes of flavonol constituents in persimmon leaves was investigated. In addition to four nongalloylated flavonol glycosides present at the leaf-shooting stage, four galloylated flavonol glycosides accumulated as leaves developed. Separation of the eight constituents was efficiently achieved through enzymatic transformation and chromatographic fractionation, and the gallates were identified as regiospecific 2″-galloylated galactosides and glucosides of kaempferol and quercetin. A mixture of the gallates had 2-fold stronger antioxidant activity than the nongallates.

    4. Metabolomics Study on Quality Control and Discrimination of Three Curcuma Species based on Gas Chromatograph–Mass Spectrometry (pages 411–418)

      Zheng Xiang, Xian-qin Wang, Xiao-jun Cai and Su Zeng

      Version of Record online: 23 MAR 2011 | DOI: 10.1002/pca.1296

      In this work, Metabolomics analysis was carried out for deliberately collected Curcuma phaeocaulis, C. kwangsiensis, and C. wenyujin samples by gas chromatography-mass spectroscopy coupled with multivariable statistical analysis. Partial least squares discrimination analysis (PLS-DA) was successfully employed to discriminate and assess the quality of three curcuma species from different ecotypes. The components contributing significantly to the discrimination were screened out and further can be used as chemical markers for discrimination and quality control of curcuma samples.

    5. Rapid Isolation and Purification of Inotodiol and Trametenolic Acid from Inonotus obliquus by High-speed Counter-current Chromatography with Evaporative Light Scatting Detection (pages 419–423)

      Deyao Du, Feng Zhu, Xianhui Chen, Xiuyun Ju, Youjian Feng, Lian-Wen Qi and Jihong Jiang

      Version of Record online: 23 MAR 2011 | DOI: 10.1002/pca.1297

      Inotodiol and trametenolic acid were rapidly isolated and purified from the chloroform extract of Inonotus obliquus (Fr.) by high-speed counter-current chromatography (HSCCC). The target compounds were finally isolated and purified with a solvent system composed of hexane-ethyl acetate-methanol-water (1:0.4:1:0.4, v/v/v/v). In a single operation, 100 mg of the extracts of I. obliquus was separated to yield 13.0 mg of inotodiol and 7.0 mg of trametenolic acid. The entire separation and purification time is less than 5 h.

    6. A Novel Protocol for the Preparation of Sodium Tanshinone Sulphonates by Direct Ultrasound-assisted Sulphonation of the Crude Extract of the Roots of Salvia miltiorrhiza Bunge and Following Counter-current Chromatography Purification (pages 424–431)

      Liping Zeng, Dingfang Wu and Shihua Wu

      Version of Record online: 4 APR 2011 | DOI: 10.1002/pca.1298

      This work developed a simple protocol for the preparation of sodium tanshinone sulphonates by direct ultrasound-assisted sulphonation of the crude extract of the roots of Tanshen (or Danshen, Salvia miltiorrihiza Bunge) and following counter-current chromatography purification without pre-purification of tanshinones. It can be explored as a new protocol for wide natural product modification directly from a crude complex extracts without pre-purification.

    7. Profiling the chlorogenic acids of Rudbeckia hirta, Helianthus tuberosus, Carlina acaulis and Symphyotrichum novae-angliae leaves by LC-MSn (pages 432–441)

      Rakesh Jaiswal, Sagar Deshpande and Nikolai Kuhnert

      Version of Record online: 14 APR 2011 | DOI: 10.1002/pca.1299

      The chlorogenic acids of four plants of the Astreraceae family, Rudbeckia hirta (black-eyed Susan), Helianthus tuberosus (Jerusalam artichoke), Carlina acaulis and Symphyotrichum novae-angliae were investigated qualitatively by LC-MSn. Fifty-two chlorogenic acids were detected and all characterised to regioisomeric level on the basis of their fragmentation pattern in the tandem MS spectra, all of them for the first time from these sources with three of them previously not reported in nature. Both chlorogenic acids based on trans- and cis-cinnamic acid substituents were identified, including mono-, di- and tri-acyl quinic acids and caffeoylshikimic acid.

    8. Simultaneous Analysis of Multiple Endogenous Plant Hormones in Leaf Tissue of Oilseed Rape by Solid-phase Extraction Coupled with High-performance Liquid Chromatography–Electrospray Ionisation Tandem Mass Spectrometry (pages 442–449)

      Yan-hua Li, Fang Wei, Xu-yan Dong, Jin-hua Peng, Sheng-yi Liu and Hong Chen

      Version of Record online: 14 APR 2011 | DOI: 10.1002/pca.1300

      A solid-phase extraction method coupled with HPLC-ESI-MS/MS was developed for determination of six plant hormones (ABA, IAA, IBA, GA3, JA and SA) in leaf tissue of oilseed rape. Under the optimal conditions, good linearities, high extraction recoveries and sensitivity and excellent reproducibility were obtained. The multiple plant hormones in leaves stimulated by oxalic acid were determined, with the results showing that these biomolecules are important for plant defence responses.

    9. One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography (pages 450–454)

      Junyou Shi, Guoliang Li, Honglun Wang, Jie Zheng, Yourui Suo, Jinmao You and Yongjun Liu

      Version of Record online: 4 APR 2011 | DOI: 10.1002/pca.1301

      By using high-speed counter-current chromatography (HSCCC) with a two-phase solvent system including n-butanol, petroleum ether and 0.5% acetic acid (5:3:5, v/v), three main flavonoids, isoquercitrin, quercetin-3-O-sophoroside and quercetin-3-O-(6′′-O-malonyl)-β-D-glucoside, were isolated from the leave of Poacynum hendersonii. The purities of the three compounds were further tested by HPLC and their structures were identified by 1H-NMR and 13C-NMR. It is proved that HSCCC is fast, simple and efficient for the preparative separation of flavonoids from the leave of Poacynum hendersonii.

    10. Simultaneous Determination of Eleven Major Flavonoids in the Pollen of Typha angustifolia by HPLC-PDA-MS (pages 455–461)

      Weiwei Tao, Nianyun Yang, Jin-ao Duan, Dekang Wu, JianMing Guo, Yuping Tang, Dawei Qian and Zhenhua Zhu

      Version of Record online: 24 MAR 2011 | DOI: 10.1002/pca.1302

      An HPLC-PDA method has been developed to simultaneously quantify the eleven major flavonoids in the pollen of Typha angustifolia Linn. The identity of the peaks was further confirmed by ESI-MS/MS. The developed method was successfully applied to the simultaneous identification and determination of 11 analytes in 16 samples of T. angustifolia collected from different places of China.

    11. Authentication Analysis of Red Fruit (Pandanus Conoideus Lam) Oil Using FTIR Spectroscopy in Combination with Chemometrics (pages 462–467)

      Abdul Rohman, Yaakob B. Che Man and Sugeng Riyanto

      Version of Record online: 24 MAR 2011 | DOI: 10.1002/pca.1304

      Red fruit (Pandanus conoideus Lam) is endemic plant of Papua, Indonesia. The objective of this research was to develop FTIR spectroscopy combined with chemometrics for authentication of red fruit oil (RFO) from canola (CaO) and rice bran oils (RBO). CaO in RFO was determined at 1200 – 1050 cm−1. The frequency ranges of 1,207 – 1,078 and 1,747 – 1,600 cm−1 were used for analysis RBO. Discriminant analysis was able to discriminate between RFO and RFO adulterated with CaO and RBO.

    12. Preparative Isolation and Purification of Seven Isoflavones from Belamcanda chinensis (pages 468–473)

      Yeon Sil Lee, Seon Ha Kim, Jin Kyu Kim, Sanghyun Lee, Sang Hoon Jung and Soon Sung Lim

      Version of Record online: 4 APR 2011 | DOI: 10.1002/pca.1306

      A high-speed countercurrent chromatography method for isolation and purification of two isoflavone glycosides and five isoflavone derivatives from the rhizomes of Belamcanda chinensis was established by using a two-step solvent system method, n-hexane/ethylacetate/2-propanol/methanol/water (5:6:2:3.5:6, v/v) and ethyl acetate/methanol/water (10:2:9, v/v). Tectoridin (145.4 mg, 97.5%), iridin (77.9 mg, 94.0%), irilin D (42.0 mg, 92.0%), tectorigenin (294.1 mg, 98.6%), iristectorigenin A (86.8 mg, 93.4%), irigenin (141.8 mg, 95.8%), and irisflorentin (73.4 mg, 94.7%) were obtained from 2 g of the crude methanol extract.

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