Phytochemical Analysis

Cover image for Vol. 24 Issue 1

January/February 2013

Volume 24, Issue 1

Pages 1–96

  1. Review

    1. Top of page
    2. Review
    3. Research Articles
    1. Application of Chemometrics in Authentication of Herbal Medicines: A Review (pages 1–24)

      Haidy A. Gad, Sherweit H. El-Ahmady, Mohamed I. Abou-Shoer and Mohamed M. Al-Azizi

      Article first published online: 7 JUN 2012 | DOI: 10.1002/pca.2378

      The use of multivariate analysis and sorting of data using various chemometric methods has become increasingly popular in the field of medicinal plants. The critical issues of herbal adulteration and extensively wide plant varieties have made quality control a sometimes difficult task that is mainly managed using different chromatographic and spectroscopic techniques in combination with chemometric analysis. In this review, a compilation of about 90 different plant species, methods of analysis used, chemometric techniques applied and the purpose of each study is discussed briefly.

  2. Research Articles

    1. Top of page
    2. Review
    3. Research Articles
    1. UPLC–PDA–ESI–MS/MS Analysis of Compounds Extracted by Cardiac h9c2 Cell from Polygonum orientale (pages 25–35)

      Shang-Gao Liao, Yue-Ting Li, Li-Juan Zhang, Zhen Wang, Teng-Xiang Chen, Yong Huang, Jing Li, Ai-Min Wang, Yong-Jun Li, Yan-Yu Lan and Yong-Lin Wang

      Article first published online: 19 JUN 2012 | DOI: 10.1002/pca.2374

      A method which couples UPLCŒPDAŒESI-MS/MS and targeted UPLCŒMRM with target cell extraction has been developed for the screening and structural characterisation of potential active compounds directly acting on the target cardiac cell. Twenty-three potentially active phenolics including ten flavonoid C-glycosides and six flavonoid O-glycosides have been identified from the 40 compounds screened in the cell extract of a flavonoid-enriched extract of Polygonum orientale. Strategies for the structural characterisation of flavonoid glycosides were also discussed.

    2. Pulsed Electric Field Extraction Enhanced Anti-coagulant Effect of Fungal Polysaccharide from Jew's Ear (Auricularia auricula) (pages 36–40)

      Changtian Li, Xinxin Mao and Baojun Xu

      Article first published online: 12 JUN 2012 | DOI: 10.1002/pca.2376

      Pulsed electric fields enhanced anticoagulant effect of Jew's ear extract. Strain DY 18 exhibited the highest anticoagulant effect. Polysaccharide from Jew's ear exhibited dose-dependent anticoagulant activity. HIPEF will be an effective method for extraction of natural components.

    3. Concentration Kinetics of Secoisolariciresinol Diglucoside and its Biosynthetic Precursor Coniferin in Developing Flaxseed (pages 41–46)

      Jingjing Fang, Aina Ramsay, Christian Paetz, Evangelos C. Tatsis, Sullivan Renouard, Christophe Hano, Eric Grand, Ophélie Fliniaux, Albrecht Roscher, Francois Mesnard and Bernd Schneider

      Article first published online: 12 JUN 2012 | DOI: 10.1002/pca.2377

      Flaxseed (Linum usitatissimum L.) is the richest source of secoisolariciresinol diglucoside (SDG) in plants. A RP-HPLC-DAD method was developed to quantify SDG and coniferin in flaxseed. Seeds harvested at different days after flowering (DAF) were subjected to alkaline hydrolysis and analysed for their SDG and coniferin concentrations. Coniferin was found when flowering started and became undetectable 20 DAF. SDG was detected 6 DAF, and the concentration increased until maturity. The biosynthesis of coniferin is asynchronous with that of SDG.

    4. Free Radical Scavenging Activities of Polyphenolic Compounds Isolated from Medicago sativa and Medicago truncatula Assessed by Means of Thin-layer Chromatography DPPH˙ Rapid Test (pages 47–52)

      Łukasz Cieśla, Iwona Kowalska, Wiesław Oleszek and Anna Stochmal

      Article first published online: 29 JUN 2012 | DOI: 10.1002/pca.2379

      A simple, rapid and reliable TLC–DPPH· test with image processing has been developed to assess the free radical scavenging activity of polyphenols isolated from Medicago sativa and Medicago truncatula. New solutions enabling better data comparison by means of an image processing program have been developed. The influence of different structural features (acylation with hydroxycinnamic acids, the amount and type of sugar moieties) on the observed activity has been studied and discussed.

    5. Monitoring of Rosmarinic Acid Accumulation in Sage Cell Cultures using Laccase Biosensor (pages 53–58)

      Sandra A. V. Eremia, Gabriel-Lucian Radu and Simona-Carmen Litescu

      Article first published online: 20 JUN 2012 | DOI: 10.1002/pca.2380

      The present work aimed the use of a previously developed laccase based biosensor for the determination of total polyphenolic content from in vitro Salvia cultures, the results being expressed as rosmarinic acid equivalent content. The response of the biosensor developed was characterised in terms of repeatability, accuracy and precision; the limit of detection was 7.5 10−7 mol/L, the limit of determination was 9.5 10−7 mol/L, and linear response range for rosmarinic acid was 10−6–10−5 mol/L.

    6. Identification of the Anti-oxidants in Flos Chrysanthemi by HPLC–DAD–ESI/MSn and HPLC Coupled with a Post-column Derivatisation System (pages 59–68)

      Yaru Niu, Liangliang Yin, Shiheng Luo, Jing Dong, Hong Wang, Yuki Hashi and Shizhong Chen

      Article first published online: 26 JUN 2012 | DOI: 10.1002/pca.2381

      A total of 17 compounds were separated and identified,3of which were identified in Jiju for the first time, and7active compounds serve as the chemical basis of theanti-oxidant efficacy of Jiju.The methods described here allow rapid separation and convenient identification of the multiple constituentsinthecomplex natural matrices.

    7. Rapid Analysis of Volatile Components from Teucrium polium L. by Nanoporous Silica-polyaniline Solid Phase Microextraction Fibre (pages 69–74)

      Mohammad Bagher Gholivand, Marzieh Piryaei, Mir Mahdi Abolghasemi and Seyed Mohammad Maassoumi

      Article first published online: 5 JUL 2012 | DOI: 10.1002/pca.2382

      A highly porous fiber coated Santa Barbara amorphous (SBA-15)/polyaniline materials were prepared for solid-phase microextraction (SPME). The proposed fiber was evaluated for the extraction of the volatile component of Teucrium polium L. The experimental results showed that the nanoporous fiber was suitable for the semi-quantitative study of the composition of essential oils in plant materials and monitoring the variations in the volatile components of the plants.

    8. Molecular Weight Determination and Correlation Analysis of Dalbergia sissoo Polysaccharide with Constituent Oligosaccharides (pages 75–80)

      Vineet Kumar, Vikas Rana and P. L. Soni

      Article first published online: 12 JUL 2012 | DOI: 10.1002/pca.2384

      Total hydrolysis and gas–liquid chromatography (GLC) analysis of alditol acetate derivatives of reduced and unreduced monosugars of Dalbergia sissoo leaf polysaccharide indicated the presence of L-rhamnose, D-glucuronic acid, D-galactose and D-glucose in 1.00:1.00:2.00:2.33 molar ratios. Partial hydrolysis followed by monosugar analysis corroborated the sugar ratio. The study established an obvious correlation of the structural oligosaccharides with polysaccharide, which is normally not studied conclusively while reporting the polysaccharide structure. The polysaccharide had an average molecular weight 1.5 × 105 Da.

    9. Multi-phase Extraction of Glycoraphanin from Broccoli Using Aminium Ionic Liquid-based Silica (pages 81–86)

      Minglei Tian, Wentao Bi and Kyung Ho Row

      Article first published online: 9 JUL 2012 | DOI: 10.1002/pca.2386

      A novel method (multi-phase extraction) was developed. The target compound from natural plant was simultaneously extracted and separated with this method. New functionalized ionic liquid-based silica was investigated. Large amount of target compound (0.038 mg g−1) was purified after 15 repeated extraction with the new sorbent. Low deviation error, small amount of required solvents, highly selective separation and stability of the method justify its for further research.

    10. Simple and Reliable Methods for the Determination of Three Steroidal Glycosides in the Eight Species of Solanum by Reversed-phase HPLC Coupled with Diode Array Detection (pages 87–92)

      Anupam Maurya, N. Manika, Ram Kishore Verma, Subhash C. Singh and Santosh Kumar Srivastava

      Article first published online: 11 JUL 2012 | DOI: 10.1002/pca.2387

      An HPLC-PDA method has been developed for the simultaneous determination of three steroidal glycosides, indioside D, solamargine and α-solanine in eight species of the genus Solanum. The method is simple, reproducible with best resolution in 16 minutes run time with one step gradient elution. The method can be successfully used for the quality control of phytopharmaceuticals containing Solanum species.

    11. Formamide-based RNA Isolation at Above ZeroTemperatures from High Starch Cassava Tubers (pages 93–96)

      Valerie Bowrin, Judy Rouse-Miller, Fedora Sutton and Grace Sirju-Charran

      Article first published online: 23 JUL 2012 | DOI: 10.1002/pca.2390

      Cassava (Manihot esculenta) tubers are a main source of carbohydrate for a large percentage of people. However, obtaining RNA from such high starch tubers proves problematic because gelation occurs during standard isolation procedures. To develop a simple, reproducible formamide-based procedure for RNA isolation from high starch tissues. Tubers were homogenized in 100 % formamide at room and centrifuged. The RNA was successfully used in downstream RT-PCR reactions. The procedure was low-cost, and removed gelation problems.

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