UPLC–PDA–ESI–MS/MS Analysis of Compounds Extracted by Cardiac h9c2 Cell from Polygonum orientale (pages 25–35)
Shang-Gao Liao, Yue-Ting Li, Li-Juan Zhang, Zhen Wang, Teng-Xiang Chen, Yong Huang, Jing Li, Ai-Min Wang, Yong-Jun Li, Yan-Yu Lan and Yong-Lin Wang
Article first published online: 19 JUN 2012 | DOI: 10.1002/pca.2374
A method which couples UPLCŒPDAŒESI-MS/MS and targeted UPLCŒMRM with target cell extraction has been developed for the screening and structural characterisation of potential active compounds directly acting on the target cardiac cell. Twenty-three potentially active phenolics including ten flavonoid C-glycosides and six flavonoid O-glycosides have been identified from the 40 compounds screened in the cell extract of a flavonoid-enriched extract of Polygonum orientale. Strategies for the structural characterisation of flavonoid glycosides were also discussed.