Both authors contributed equally to the manuscript.
In vivo model to determine fetal-cell enrichment efficiency of novel noninvasive prenatal diagnosis methods
Article first published online: 28 MAY 2008
Copyright © 2008 John Wiley & Sons, Ltd.
Volume 28, Issue 6, pages 494–502, June 2008
How to Cite
Ponnusamy, S., Mohammed, N., Ho, S. S. Y., Zhang, H. M., Chan, Y. H., Ng, Y. W., Su, L. L., Mahyuddin, A. P., Venkat, A., Chan, J., Rauff, M., Biswas, A. and Choolani, M. (2008), In vivo model to determine fetal-cell enrichment efficiency of novel noninvasive prenatal diagnosis methods. Prenat. Diagn., 28: 494–502. doi: 10.1002/pd.2009
- Issue published online: 28 MAY 2008
- Article first published online: 28 MAY 2008
- Manuscript Accepted: 14 MAR 2008
- Manuscript Revised: 3 MAR 2008
- Manuscript Received: 13 DEC 2007
- National Medical Research Council. Grant Number: NMRC Grant No. 0561/2001
- fetal primitive erythroblasts;
- maternal blood;
- in vivo model
To develop an in vivo model to determine fetal-cell enrichment efficiency of novel noninvasive prenatal diagnosis methods.
Efficiency of our three-step enrichment protocol was determined in vitro before fetal nucleated red blood cells (FNRBCs) were enriched from first-trimester maternal blood samples collected from the same patients pre- and postsurgical termination of pregnancy (TOP) (n = 10). FNRBCs enriched were identified using embryonic ε-globin immunocytochemistry and chromosomal fluorescence in situ hybridization.
We recovered 37% of spiked FNRBCs (95% confidence interval (CI) 28.5–45.6; n = 8) in in vitro experiments. We show a consistent threefold increase in the number of ε + FNRBCs in maternal blood obtained immediately post-TOP (p = 0.005). A mathematical relationship was derived: observed number of pretermination primitive FNRBCs = 0.6 + 0.31 (coefficient between pretermination/post-termination primitive FNRBCs, 95% CI 0.12–0.49; p = 0.005) × observed number of post-termination primitive FNRBCs (R2 = 0.65).
Our data demonstrate that maternal blood obtained immediately post-TOP would be a good in vivo model to determine the enrichment efficiency of novel protocols and methods for noninvasive prenatal diagnosis. Copyright © 2008 John Wiley & Sons, Ltd.