Funding sources: Our work on tissue engineering in perinatology including membrane wound healing is funded by the European Commission (EC) via the EuroSTEC project (LSHC-CT-2006-037409). M. E. is a beneficiary of a Marie Curie Industry-Academia Partnership and Pathways grant from the EC (251356). J. P. D. is a beneficiary of a fundamental clinical research grant from the Fonds Wetenschappelijk Onderzoek Vlaanderen (1.8.012.07.N.02). J. R. is a beneficiary of a grant from the KOOR Fonds of the University Hospitals Leuven.
In vitro sealing of iatrogenic fetal membrane defects by a collagen plug imbued with fibrinogen and plasma
Article first published online: 7 JAN 2013
© 2013 John Wiley & Sons, Ltd.
Volume 33, Issue 2, pages 162–167, February 2013
How to Cite
Engels, A. C., Hoylaerts, M. F., Endo, M., Loyen, S., Verbist, G., Manodoro, S., DeKoninck, P., Richter, J. and Deprest, J. A. (2013), In vitro sealing of iatrogenic fetal membrane defects by a collagen plug imbued with fibrinogen and plasma. Prenat. Diagn., 33: 162–167. doi: 10.1002/pd.4032
Conflicts of interest: None declared
- Issue published online: 3 FEB 2013
- Article first published online: 7 JAN 2013
We aimed to demonstrate local thrombin generation by fetal membranes, as well as its ability to generate fibrin from fibrinogen concentrate. Furthermore, we aimed to investigate the efficacy of collagen plugs, soaked with plasma and fibrinogen, to seal iatrogenic fetal membrane defects.
Thrombin generation by homogenized fetal membranes was measured by calibrated automated thrombography. To identify the coagulation caused by an iatrogenic membrane defect, we analyzed fibrin formation by optical densitometry, upon various concentrations of fibrinogen. The ability of a collagen plug soaked with fibrinogen and plasma was tested in an ex vivo model for its ability to seal an iatrogenic fetal membrane defect.
Fetal membrane homogenates potently induced thrombin generation in amniotic fluid and diluted plasma. Upon the addition of fibrinogen concentrate, potent fibrin formation was triggered. Measured by densiometry, fibrin formation was optimal at 1250 µg/mL fibrinogen in combination with 4% plasma. A collagen plug soaked with fibrinogen and plasma sealed an iatrogenic membrane defect about 35% better than collagen plugs without these additives (P = 0.037).
These in vitro experiments suggest that the addition of fibrinogen and plasma may enhance the sealing efficacy of collagen plugs in closing iatrogenic fetal membrane defects. © 2013 John Wiley & Sons, Ltd.