Magnetic-activated cell sorting (MACS) significantly decreases the hybridization efficiency of fluorescence in situ hybridization (FISH)

Authors

  • Pao-Lin Kuo,

    Corresponding author
    1. Department of Obstetrics & Gynecology, National Cheng-Kung University Medical College, Tainan, Taiwan, ROC
    • Department of Obstetrics & Gynecology, National Cheng-Kung University Hospital, 138 Sheng-Li Road, Tainan, Taiwan 70428, ROC.
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  • How-Ran Guo

    1. Department of Environmental & Occupational Health, National Cheng-Kung University Medical College, Tainan, Taiwan, ROC
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Abstract

Fetal cells were enriched from maternal blood using density gradient centrifugation of Histopaque followed by magnetic-activated cell sorting (MACS) to select CD71-positive cells. For each specimen, cells partially purified by Histopaque were split into equal portions, and each portion was subjected to purification by MACS in parallel. Cells before and after MACS were subjected to dual-color fluorescence in situ hybridization (FISH) analysis with X- and Y-chromosome-specific probes. We found that the hybridization rates were decreased by approximately 10% after MACS based on duplicated analysis for each sample. Copyright © 2001 John Wiley & Sons, Ltd.

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