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Preparation and characterization of polyhydroxybutyrate-co-hydroxyvalerate/silk fibroin nanofibrous scaffolds for skin tissue engineering

Authors

  • Caihong Lei,

    1. Key Laboratory of Fiber Materials and Processing Technology, Zhejiang Sci-Tech University, Hangzhou, China
    2. Engineering Research Center for Eco-Dyeing & Finishing of Textiles, Ministry of Education, Zhejiang Sci-Tech University, Hangzhou, China
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  • Hailin Zhu,

    Corresponding author
    1. Key Laboratory of Fiber Materials and Processing Technology, Zhejiang Sci-Tech University, Hangzhou, China
    2. Engineering Research Center for Eco-Dyeing & Finishing of Textiles, Ministry of Education, Zhejiang Sci-Tech University, Hangzhou, China
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  • Jingjing Li,

    1. Key Laboratory of Fiber Materials and Processing Technology, Zhejiang Sci-Tech University, Hangzhou, China
    2. Engineering Research Center for Eco-Dyeing & Finishing of Textiles, Ministry of Education, Zhejiang Sci-Tech University, Hangzhou, China
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  • Jiuming Li,

    1. Key Laboratory of Fiber Materials and Processing Technology, Zhejiang Sci-Tech University, Hangzhou, China
    2. Engineering Research Center for Eco-Dyeing & Finishing of Textiles, Ministry of Education, Zhejiang Sci-Tech University, Hangzhou, China
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  • Xinxing Feng,

    1. The Quartermaster Research Institute of the General Logistic Department of CPLA, Beijing, China
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  • Jianyong Chen

    1. Key Laboratory of Fiber Materials and Processing Technology, Zhejiang Sci-Tech University, Hangzhou, China
    2. Engineering Research Center for Eco-Dyeing & Finishing of Textiles, Ministry of Education, Zhejiang Sci-Tech University, Hangzhou, China
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Abstract

Nanofibrous scaffolds were obtained by co-electrospinning poly (3-hydroxybuty-rate-co-3-hydroxyvalerate) (PHBV) and fibroin regenerated from silk in different proportions using 1,1,1,3,3,3-hexafluoro-2-isopropanol (HFIP) as solvent. Field emission scanning electron microscope (FESEM) investigation showed that the fiber diameters of the nanofibrous scaffolds ranged from 190 to 460 nm. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy analysis (FT-IR) showed that the main structure of silk fibroin (SF) in the nanofibrous scaffold was β-sheet. Compared to the PHBV nanofibrous scaffold, the surface hydrophilicity and water-uptake capability of the PHBV/SF nanofibrous scaffold with 50/50 were improved. The results of cell adhesion experiment showed that the fibroblasts adhered more to the PHBV/SF nanofibrous scaffold with 50/50 than the pure PHBV nanofibrous scaffold. The proliferation of fibroblast on the PHBV/SF nanofibrous scaffold with 50/50 was higher than that on the pure PHBV nanofibrous scaffold. Our results indicated that the PHBV/SF nanofibrous scaffold with 50/50 may be a better candidate for biomedical applications such as skin tissue engineering and wound dressing. POLYM. ENG. SCI., 55:907–916, 2015. © 2014 Society of Plastics Engineers

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