Politically conservative (versus liberal) individuals generally report more prejudice towards various low-status out-groups. Three studies examined whether prejudice suppression factors—specifically, internal and external motivation to suppress (IMS and EMS, respectively) prejudice—can help explain the relationship between political orientation and prejudice. Study 1 showed that IMS and EMS partially mediated the relationship between political orientation and affective prejudice towards Arabs. Study 2 demonstrated that when justification [right-wing authoritarianism (RWA) and social dominance orientation] and suppression (IMS and EMS) factors are simultaneously tested as mediators, only RWA partially mediated the relationship between political orientation and prejudice towards deviant (e.g. gay men) out-groups, whereas RWA and IMS fully mediated the relationship between political orientation and prejudice towards derogated out-groups (e.g. Blacks). Intriguingly, IMS rendered social dominance orientation effects non-significant for derogated out-groups. Study 3 showed that anticipating an out-group interaction (with a Black or lesbian confederate) diminished the mediational contribution of IMS in the political orientation–prejudice relationship because of increased IMS among participants; yet the increases in IMS did not completely eliminate differences in prejudice as a function of political orientation. Ultimately, these three studies demonstrate that suppression (in addition to justification) factors do help explain the relationship between political orientation and prejudice. Copyright © 2013 European Association of Personality Psychology.