Novel hydrogels based on methacrylate-modified agarose

Authors

  • Ilse De Paepe,

    1. University of Ghent, Department of Organic Chemistry, Polymer Materials Research Group, Institute of Biomedical Technologies (IBITECH), Krijgslaan 281, S4-bis, B-9000 Ghent, Belgium
    Search for more papers by this author
  • Heidi Declercq,

    1. University of Ghent, Department of Organic Chemistry, Polymer Materials Research Group, Institute of Biomedical Technologies (IBITECH), Krijgslaan 281, S4-bis, B-9000 Ghent, Belgium
    Search for more papers by this author
  • Maria Cornelissen,

    1. University of Ghent, Department of Histology, Polymer Materials Research Group, Institute of Biomedical Technologies (IBITECH), Krijgslaan 281, S4-bis, B-9000 Ghent, Belgium
    Search for more papers by this author
  • Etienne Schacht

    Corresponding author
    1. University of Ghent, Department of Organic Chemistry, Polymer Materials Research Group, Institute of Biomedical Technologies (IBITECH), Krijgslaan 281, S4-bis, B-9000 Ghent, Belgium
    • University of Ghent, Department of Organic Chemistry, Polymer Materials Research Group, Institute of Biomedical Technologies (IBITECH), Krijgslaan 281, S4-bis, B-9000, Ghent, Belgium
    Search for more papers by this author

  • Oral presentation – Session A: Paper presented at the Polymers in the Third Millennium Conference, 2–6 September 2001, Montpellier, France

Abstract

Hydrogels based on agarose were prepared aiming to obtain flexible, firm and transparent materials suitable for biochemical applications. The kinetics of gelation were studied by means of rheology. Agarose was chemically modified by introduction of methacrylate side-groups. The latter can be crosslinked to result in a network structure. The gel formation of the modified agarose is a combinatioin of physical gelation and chemical crosslinkage. The water-soluble agarose obtained after derivatization was crosslinked by UV-irradiation in the presence of a water soluble photoinitiator. The effect of storage time before chemical crosslinking has been examined and has a large influence on the final storage modulus and the contribution of the chemical and physical network strength towards the total network strength. The influence of the degree of modification on the final mechanical properties of the material has been examined by texturometry.

© 2002 Society of Chemical Industry

Ancillary