Research Article

Differential protein expression assessed by two-dimensional gel electrophoresis for two wheat varieties grown at four nitrogen levels

  1. Nasser Bahrman1,*,
  2. Jacques Le Gouis1,
  3. Luc Negroni2,
  4. Laurence Amilhat3,
  5. Philippe Leroy3,
  6. Anne-Lyse Lainé1,
  7. Odile Jaminon1

Article first published online: 2 FEB 2004

DOI: 10.1002/pmic.200300571

Issue

PROTEOMICS

PROTEOMICS

Volume 4, Issue 3, pages 709–719, March 2004

Additional Information

How to Cite

Bahrman, N., Le Gouis, J., Negroni, L., Amilhat, L., Leroy, P., Lainé, A.-L. and Jaminon, O. (2004), Differential protein expression assessed by two-dimensional gel electrophoresis for two wheat varieties grown at four nitrogen levels. PROTEOMICS, 4: 709–719. doi: 10.1002/pmic.200300571

Author Information

  1. 1

    INRA, Domaine de Brunehaut, Estrées – Mons, France

  2. 2

    UMR de Génétique Végétale, Ferme du Moulon, Gif-sur-Yvette, France

  3. 3

    UMR INRA-UBP ASP Clermont-Ferrand, France

*INRA, Unité de Recherche en Génétique et Amélioration des Plantes, Domaine de Brunehaut, F-80200 Estrées – Mons, France Fax: +33-3-2285-6996

Publication History

  1. Issue published online: 19 FEB 2004
  2. Article first published online: 2 FEB 2004
  3. Manuscript Received: 27 FEB 2003

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Keywords:

  • Genotype x nitrogen level interaction;
  • Nitrogen stress;
  • Proteome;
  • Triticum aestivum L.

Abstract

To limit N-fertilizer applied on wheat, cultivars that use N more efficiently are needed. Our objective was to investigate differences of nitrogen utilization in varieties by studying qualitative and quantitative proteins expression. Two wheat varieties, ‘Arche’ and ‘Récital’, were grown under controlled conditions at four N levels (0, 2, 8, and 20 mg N/plant/day) with two replicates. The number of tillers/plant, aerial dry weight/plant and total N content were measured after two months. Two-dimensional gel electrophoresis was also performed on leaf protein extracts. Analyses of variance showed that the N level effect was highly significant for the number of tillers/plant, aerial dry weight and N content. The variety×N level interaction was significant for N content. Analyses of variance on % volume carried out for 524 spots showed a significant variety effect for 55 spots and a significant N treatment effect for 76 spots. Twenty spots showed a significant variety×N treatment interaction. Fourteen proteins were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The possible role of these proteins, eight of which belong to the carbon metabolism, is discussed.

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