Research Article

Photoaptamer arrays applied to multiplexed proteomic analysis

  1. Chris Bock,
  2. Mike Coleman,
  3. Brian Collins,
  4. Jody Davis,
  5. Glenn Foulds,
  6. Larry Gold,
  7. Chad Greef,
  8. Jim Heil,
  9. Joseph S. Heilig,
  10. Brian Hicke,
  11. Michele Nelson Hurst,
  12. Gregory M. Husar,
  13. Darcey Miller,
  14. Rachel Ostroff,
  15. Helen Petach*,
  16. Dan Schneider,
  17. Barry Vant-Hull,
  18. Sheela Waugh,
  19. Allison Weiss,
  20. Sheri K. Wilcox,
  21. Dominic Zichi

Article first published online: 2 FEB 2004

DOI: 10.1002/pmic.200300631

Issue

PROTEOMICS

PROTEOMICS

Volume 4, Issue 3, pages 609–618, March 2004

Additional Information

How to Cite

Bock, C., Coleman, M., Collins, B., Davis, J., Foulds, G., Gold, L., Greef, C., Heil, J., Heilig, J. S., Hicke, B., Nelson Hurst, M., Husar, G. M., Miller, D., Ostroff, R., Petach, H., Schneider, D., Vant-Hull, B., Waugh, S., Weiss, A., Wilcox, S. K. and Zichi, D. (2004), Photoaptamer arrays applied to multiplexed proteomic analysis. PROTEOMICS, 4: 609–618. doi: 10.1002/pmic.200300631

Author Information

  1. SomaLogic, Boulder, CO, USA

*SomaLogic, 1745 38th St., Boulder, CO 80301, USA Fax: +1-303-545-2525

Publication History

  1. Issue published online: 19 FEB 2004
  2. Article first published online: 2 FEB 2004
  3. Manuscript Received: 19 MAY 2003

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Keywords:

  • Aptamers;
  • Multiplex array;
  • Photoaptamer;
  • Protein microarray

Abstract

Multiplexed photoaptamer-based arrays that allow for the simultaneous measurement of multiple proteins of interest in serum samples are described. Since photoaptamers covalently bind to their target analytes before fluorescent signal detection, the arrays can be vigorously washed to remove background proteins, providing the potential for superior signal-to-noise ratios and lower limits of quantification in biological matrices. Data are presented here for a 17-plex photoaptamer array exhibiting limits of detection below 10 fM for several analytes including interleukin-16, vascular endothelial growth factor, and endostatin and able to measure proteins in 10% serum samples. The assays are simple, scalable, and reproducible. Affinity of the capture reagent is shown to be directly correlated to the limit of detection for the analyte on the array.

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